Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of relevant immunogens that can be used for the development of a new generation of vaccines against a variety of infectious diseases. In the present study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108-120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The fusion protein L2 108-120-PVX CP was successfully expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The C-terminal fusion protein PVX CP- L2 108-120 was expressed using mutated vector sequence to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2 108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2 108-120 epitope were found after both methods of vaccine delivery.

• MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• ELISA MeSH
• epitopy genetika   metabolismus   MeSH
• genetické vektory genetika   MeSH
• geneticky modifikované rostliny MeSH
• imunizace MeSH
• klonování DNA MeSH
• lidé MeSH
• listy rostlin metabolismus   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• oligonukleotidy genetika   MeSH
• onkogenní proteiny virové metabolismus   MeSH
• protilátky virové krev   MeSH
• rekombinantní fúzní proteiny imunologie   metabolismus   MeSH
• tabák metabolismus   MeSH
• transmisní elektronová mikroskopie MeSH
• virion imunologie   MeSH
• virové plášťové proteiny metabolismus   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• coat protein, Potato virus X MeSH Prohlížeč
• epitopy MeSH
• L2 protein, Human papillomavirus type 16 MeSH Prohlížeč
• oligonukleotidy MeSH
• onkogenní proteiny virové MeSH
• protilátky virové MeSH
• rekombinantní fúzní proteiny MeSH
• virové plášťové proteiny MeSH