The aim of the current study was to screen and identify heavy metal (chromium, cadmium, and lead) associated bacteria from petroleum-contaminated soil of district Muzaffarabad, Azad Jammu and Kashmir, Pakistan to develop ecofriendly technology for contaminated soil remediation. The petroleum-contaminated soil was collected from 99 different localities of district Muzaffarabad and the detection of heavy metals via an atomic absorption spectrometer. The isolation and identification of heavy metals-associated bacteria were done via traditional and molecular methods. Resistogram and antibiogram analysis were also performed using agar well diffusion and agar disc diffusion methods. The isolated bacteria were classified into species, i.e., B. paramycoides, B. albus, B. thuringiensis, B. velezensis, B. anthracis, B. pacificus Burkholderia arboris, Burkholderia reimsis, Burkholderia aenigmatica, and Streptococcus agalactiae. All heavy metals-associated bacteria showed resistance against both high and low concentrations of chromium while sensitive towards high and low concentrations of lead in the range of 3.0 ± 0.0 mm to 13.0 ± 0.0 mm and maximum inhibition was recorded when cadmium was used. Results revealed that some bacteria showed sensitivity towards Sulphonamides, Norfloxacin, Erythromycin, and Tobramycin. It was concluded that chromium-resistant bacteria could be used as a favorable source for chromium remediation from contaminated areas and could be used as a potential microbial filter.

• Klíčová slova
• Bacillus spp, Antibiogram analysis, Heavy metals, Petroleum contaminated soil, Resistogram analysis, Ribotyping,
• MeSH
• antibakteriální látky * farmakologie   MeSH
• Bacteria * účinky léků   klasifikace   izolace a purifikace   genetika   MeSH
• chrom metabolismus   MeSH
• kadmium analýza   MeSH
• látky znečišťující půdu * analýza   MeSH
• mikrobiální testy citlivosti * MeSH
• olovo MeSH
• půda chemie   MeSH
• půdní mikrobiologie * MeSH
• ropa mikrobiologie   analýza   MeSH
• těžké kovy * farmakologie   analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Pákistán MeSH
• Názvy látek
• antibakteriální látky * MeSH
• chrom MeSH
• kadmium MeSH
• látky znečišťující půdu * MeSH
• olovo MeSH
• půda MeSH
• ropa MeSH
• těžké kovy * MeSH

Papiliotrema huenov was previously reported to be highly tolerant of a range of extremely toxic heavy metals. This study aimed to identify the potential of P. huenov to remove manganese and copper from aqueous solution. Physical conditions which affect removal of Mn(II) and Cu(II) were determined. Optimal temperature for adsorption of both metal ions was 30 °C, and optimal pH for maximum uptake of Mn(II) and Cu(II) were 5 and 6, respectively. Under these conditions, living cells of P. huenov accumulated up to 75.58% of 110 mg/L Mn(II) and 70.5% of 128 mg/L Cu(II) over 120 h, whereas, the removal efficiency of metal ions by dead cells over 1 h was 60.3% and 56.5%, respectively. These results indicate that living cells are more effective than dead biomass for bioremediation, but that greater time is required. The experimental data extends the potential use of P. huenov in biosorption and bioaccumulation of toxic heavy metals to copper and manganese, two of the most common industrial contaminants.

• Klíčová slova
• Papiliotrema huenov, bioaccumulation, biosorption, copper, manganese,
• Publikační typ
• časopisecké články MeSH