Most cited article - PubMed ID 21425677
Determination of oxygen radical absorbance capacity of black cumin (Nigella sativa) seed quinone compounds
In this study, a new broth macrodilution volatilization method for the simple and rapid determination of the antibacterial effect of volatile agents simultaneously in the liquid and vapor phase was designed with the aim to assess their therapeutic potential for the development of new inhalation preparations. The antibacterial activity of plant volatiles (β-thujaplicin, thymohydroquinone, thymoquinone) was evaluated against bacteria associated with respiratory infections (Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes) and their cytotoxicity was determined using a modified thiazolyl blue tetrazolium bromide assay against normal lung fibroblasts. Thymohydroquinone and thymoquinone possessed the highest antibacterial activity against H. influenzae, with minimum inhibitory concentrations of 4 and 8 µg/mL in the liquid and vapor phases, respectively. Although all compounds exhibited cytotoxic effects on lung cells, therapeutic indices (TIs) suggested their potential use in the treatment of respiratory infections, which was especially evident for thymohydroquinone (TI > 34.13). The results demonstrate the applicability of the broth macrodilution volatilization assay, which combines the principles of broth microdilution volatilization and standard broth macrodilution methods. This assay enables rapid, simple, cost- and labor-effective screening of volatile compounds and overcomes the limitations of assays currently used for screening of antimicrobial activity in the vapor phase.
- Keywords
- antimicrobial, cytotoxicity, macrodilution method, respiratory infections, thymohydroquinone, thymoquinone, vapor phase, volatile compound, β-thujaplicin,
- MeSH
- Anti-Bacterial Agents analysis chemistry pharmacology MeSH
- Administration, Inhalation MeSH
- Bacteria drug effects MeSH
- Benzoquinones administration & dosage pharmacology MeSH
- Haemophilus influenzae drug effects MeSH
- Microbial Sensitivity Tests MeSH
- Monoterpenes administration & dosage pharmacology MeSH
- Oils, Volatile chemistry pharmacology MeSH
- Staphylococcus aureus drug effects MeSH
- Streptococcus pneumoniae drug effects MeSH
- Streptococcus pyogenes drug effects MeSH
- Volatile Organic Compounds chemistry pharmacology MeSH
- Thymol administration & dosage analogs & derivatives pharmacology MeSH
- Tropolone administration & dosage analogs & derivatives pharmacology MeSH
- Volatilization MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Benzoquinones MeSH
- beta-thujaplicin MeSH Browser
- Monoterpenes MeSH
- Oils, Volatile MeSH
- Volatile Organic Compounds MeSH
- thymohydroquinone MeSH Browser
- Thymol MeSH
- thymoquinone MeSH Browser
- Tropolone MeSH