The biochemical basis of a defective bioenergetic system was attempted to be determined in N,N'-dicyclohexylcarbodiimide (DCCD)-resistant mutant of Methanothermobacter thermautotrophicus. Components participating in the maintenance of methanoarchaeal membrane structure and function, such as the composition of the mixture of squalene and its hydrosqualene derivatives and also properties of membrane-associated proteins were compared in wild-type and mutant cells. The impairment of the bioenergetic system in DCCD-resistant mutant was detectable in the membrane-protein profile; it was also accompanied by changes in proportions of squalene-hydrosqualenes.

• MeSH
• antibiotická rezistence * MeSH
• buněčná membrána metabolismus   MeSH
• dicyklohexylkarbodiimid metabolismus   farmakologie   MeSH
• energetický metabolismus MeSH
• membránové proteiny genetika   metabolismus   MeSH
• Methanobacteriaceae účinky léků   genetika   metabolismus   MeSH
• mutace MeSH
• skvalen chemie   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dicyklohexylkarbodiimid MeSH
• membránové proteiny MeSH
• skvalen MeSH

In an attempt to more closely define a protein basis of differences in ATPase and ATP synthase activities in a mutant of the methanoarchaeon Methanothermobacter thermautotrophicus resistant to the protonophoric uncoupler TCS (3,3',4',5-tetrachlorosalicylanilide), the composition of membrane associated proteins from the wild-type and mutant strains has been compared. The uncoupler-resistance in the mutant strain was not accompanied by changes in a protein size or changes in the level of subunits A, B and c (proteolipid) of the A1A0-type ATPase-synthase. On the other hand, we revealed a 670-kDa membrane-associated protein complex that is abundantly present only in the mutant strain; it is composed of at least 5 different subunits of 95, 52, 42, 29 and 22 kDa.

• MeSH
• antibiotická rezistence genetika   MeSH
• archeální proteiny analýza   izolace a purifikace   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• inhibitory enzymů farmakologie   MeSH
• membránové proteiny analýza   izolace a purifikace   MeSH
• Methanobacteriaceae účinky léků   enzymologie   genetika   MeSH
• molekulová hmotnost MeSH
• mutace * MeSH
• podjednotky proteinů analýza   izolace a purifikace   MeSH
• protonové ATPasy analýza   izolace a purifikace   MeSH
• salicylanilidy farmakologie   MeSH
• western blotting MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 3,3',4',5-tetrachlorosalicylanilide MeSH Prohlížeč
• archeální proteiny MeSH
• inhibitory enzymů MeSH
• membránové proteiny MeSH
• podjednotky proteinů MeSH
• protonové ATPasy MeSH
• salicylanilidy MeSH