In an attempt to more closely define a protein basis of differences in ATPase and ATP synthase activities in a mutant of the methanoarchaeon Methanothermobacter thermautotrophicus resistant to the protonophoric uncoupler TCS (3,3',4',5-tetrachlorosalicylanilide), the composition of membrane associated proteins from the wild-type and mutant strains has been compared. The uncoupler-resistance in the mutant strain was not accompanied by changes in a protein size or changes in the level of subunits A, B and c (proteolipid) of the A1A0-type ATPase-synthase. On the other hand, we revealed a 670-kDa membrane-associated protein complex that is abundantly present only in the mutant strain; it is composed of at least 5 different subunits of 95, 52, 42, 29 and 22 kDa.

• MeSH
• antibiotická rezistence genetika   MeSH
• archeální proteiny analýza   izolace a purifikace   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• inhibitory enzymů farmakologie   MeSH
• membránové proteiny analýza   izolace a purifikace   MeSH
• Methanobacteriaceae účinky léků   enzymologie   genetika   MeSH
• molekulová hmotnost MeSH
• mutace * MeSH
• podjednotky proteinů analýza   izolace a purifikace   MeSH
• protonové ATPasy analýza   izolace a purifikace   MeSH
• salicylanilidy farmakologie   MeSH
• western blotting MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 3,3',4',5-tetrachlorosalicylanilide MeSH Prohlížeč
• archeální proteiny MeSH
• inhibitory enzymů MeSH
• membránové proteiny MeSH
• podjednotky proteinů MeSH
• protonové ATPasy MeSH
• salicylanilidy MeSH

The effect of ethidium bromide on the growth of a yeast mutant with an impaired mitochrondrial translocation system of adenine nucleotides (op-1 mutant) was investigated. It was found that the op-1 mutant stops growing both under growing and non-growing conditions after treatment with ethidium bromide and that the growth cannot be restored by adding low-molecular compounds to the growth medium. It was the aim of the experiments to clarify whether the cessation of growth of the op-1 mutant after induction of the rho- mutation can be simulated by inhibitors phenotypically changing the mitochondrial function. It appears likely that the op-1 mutant stops growing only after the rho- mutation has been induced, because the phenotypic simulation of the rho- mutation does not lead the cessation of growth of the op-1 mutant.

• MeSH
• adeninnukleotidy metabolismus   MeSH
• antibakteriální látky farmakologie   MeSH
• buněčné dělení MeSH
• ethidium farmakologie   MeSH
• kultivační média MeSH
• kvasinky účinky léků   růst a vývoj   metabolismus   MeSH
• mitochondrie metabolismus   MeSH
• mutace * MeSH
• mutageny MeSH
• spotřeba kyslíku MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adeninnukleotidy MeSH
• antibakteriální látky MeSH
• ethidium MeSH
• kultivační média MeSH
• mutageny MeSH