The aim of this work was to express the recombinant hexon protein of the hemorrhagic enteritis virus, to establish the diagnostic value of this protein for serological detection of antibodies in turkey serum samples and to assess seroprevalence of the infection in the Czech Republic. The N' terminal part of the hexon protein was expressed in a bacterial expression system and used as an antigen in an ELISA test for the detection of hemorrhagic enteritis virus specific antibodies in turkey sera. Validation of the test was performed by comparison with a commercially available ELISA test. Serological reactivity was assessed on a panel of 126 turkey sera by a newly developed ELISA test. Serum samples were taken from turkey farms with the history of hemorrhagic enteritis virus infection, from farms with animals free of infection, and from turkey farms following vaccination. Both ELISA kits gave identical results (100 %) with the tested sera. ELISA based on the recombinant hexon protein thus proved useful and cheaper for detection of antibodies in turkey flocks infected with the hemorrhagic enteritis virus.

• MeSH
• Adenoviridae klasifikace   genetika   imunologie   MeSH
• antigeny virové genetika   imunologie   MeSH
• ELISA MeSH
• exprese genu * MeSH
• krocani MeSH
• protilátky virové krev   imunologie   MeSH
• rekombinantní proteiny genetika   imunologie   izolace a purifikace   MeSH
• specificita protilátek imunologie   MeSH
• virové plášťové proteiny genetika   imunologie   izolace a purifikace   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• hexon capsid protein, Adenovirus MeSH Prohlížeč
• protilátky virové MeSH
• rekombinantní proteiny MeSH
• virové plášťové proteiny MeSH