Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described. After gonadotropin stimulus, cumulus cells expand and form hyaluronan (HA)-rich cumulus ECM. In pigs, the proper structure of the cumulus ECM depends on the interaction between HA and serum-derived proteins of the inter-alpha-trypsin inhibitor (IαI) protein family. We have demonstrated the synthesis of HA by cumulus cells, and the presence of the IαI, tumor necrosis factor-alpha-induced protein 6 and pentraxin 3 in expanding oocyte-cumulus complexes (OCC). We have evaluated the covalent linkage of heavy chains of IαI proteins to HA, as the principal component of the expanded HA-rich cumulus ECM, in porcine OCC cultured in medium with specific inhibitors: AG1478 and lapatinib (both inhibitors of epidermal growth factor receptor tyrosine kinase activity); MG132 (a specific proteasomal inhibitor), indomethacin (cyclooxygenase inhibitor); and progesterone receptor antagonist (RU486). We have found that both RU486 and indomethacin does not disrupt the formation of the covalent linkage between the heavy chains of IαI to HA in the expanded OCC. In contrast, the inhibitors AG1478 and lapatinib prevent gonadotropin-induced cumulus expansion. Finally, the formation of oocyte-cumulus ECM relying on the covalent transfer of heavy chains of IαI molecules to HA has been inhibited in the presence of MG132.

• Klíčová slova
• extracellular matrix, hyaluronan, inter-alpha-trypsin inhibitor, oocyte-cumulus complexes, pentraxin 3, tumor necrosis factor-alpha-induced protein 6,
• MeSH
• C-reaktivní protein metabolismus   MeSH
• extracelulární matrix účinky léků   metabolismus   MeSH
• kumulární buňky cytologie   účinky léků   metabolismus   MeSH
• kyselina hyaluronová metabolismus   MeSH
• mifepriston farmakologie   MeSH
• molekuly buněčné adheze metabolismus   MeSH
• oocyty cytologie   metabolismus   MeSH
• rozmnožování účinky léků   MeSH
• sérový amyloidový protein metabolismus   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• C-reaktivní protein MeSH
• kyselina hyaluronová MeSH
• mifepriston MeSH
• molekuly buněčné adheze MeSH
• PTX3 protein MeSH Prohlížeč
• sérový amyloidový protein MeSH
• Tnfaip6 protein, mouse MeSH Prohlížeč