BACKGROUND: Since many acutely admitted older adults display signs of dehydration, treatment using balanced crystalloids is an important part of medical care. Additionally, many of these patients suffer from chronic malnutrition. We speculated that the early addition of glucose might ameliorate the hospital-related drop of caloric intake and modify their catabolic status. METHODS: We included patients 78 years and older, admitted acutely for non-traumatic illnesses. The patients were randomized into either receiving balanced crystalloid (PlasmaLyte; group P) or balanced crystalloid enriched with 100 g of glucose per liter (group G). The information about fluid balance and levels of minerals were collected longitudinally. RESULTS: In the G group, a significantly higher proportion of patients developed signs of refeeding syndrome, i.e., drops in phosphates, potassium and/or magnesium when compared to group P (83.3 vs. 16.7%, p < 0.01). The drop in phosphate levels was the most pronounced. The urinalysis showed no differences in the levels of these minerals in the urine, suggesting their uptake into the cells. There were no differences in the in-hospital mortality or in the 1-year mortality. CONCLUSION: The short-term administration of balanced crystalloids with glucose induced an anabolic shift of electrolytes in acutely admitted older adults.

• Klíčová slova
• dehydration, glucose, malnutrition, refeeding syndrome,
• MeSH
• dehydratace terapie   MeSH
• glukosa * metabolismus   aplikace a dávkování   MeSH
• krystaloidní roztoky aplikace a dávkování   MeSH
• lidé MeSH
• mortalita v nemocnicích MeSH
• potravní doplňky MeSH
• realimentační syndrom prevence a kontrola   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• tekutinová terapie * metody   MeSH
• vodní a elektrolytová rovnováha MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• glukosa * MeSH
• krystaloidní roztoky MeSH

Methyltriphenylphosphonium (TPMP) salts have been widely used to measure the mitochondrial membrane potential and the triphenylphosphonium (TPP+) moiety has been attached to many bioactive compounds including antioxidants to target them into mitochondria thanks to their high affinity to accumulate in the mitochondrial matrix. The adverse effects of these compounds on cellular metabolism have been insufficiently studied and are still poorly understood. Micromolar concentrations of TPMP cause a progressive inhibition of cellular respiration in adherent cells without a marked effect on mitochondrial coupling. In permeabilized cells the inhibition was limited to NADH-linked respiration. We found a mixed inhibition of the Krebs cycle enzyme 2-oxoglutarate dehydrogenase complex (OGDHC) with an estimated IC50 3.93 [3.70-4.17] mM, which is pharmacologically plausible since it corresponds to micromolar extracellular concentrations. Increasing the lipophilic character of the used TPP+ compound further potentiates the inhibition of OGDHC activity. This effect of TPMP on the Krebs cycle ought to be taken into account when interpreting observations on cells and mitochondria in the presence of TPP+ derivatives. Compounds based on or similar to TPP+ derivatives may also be used to alter OGDHC activity for experimental or therapeutic purposes.

• MeSH
• buněčné linie MeSH
• citrátový cyklus účinky léků   MeSH
• citrátsynthasa účinky léků   metabolismus   MeSH
• glutamátdehydrogenasa účinky léků   metabolismus   MeSH
• isocitrátdehydrogenasa účinky léků   metabolismus   MeSH
• ketoglutarátdehydrogenasový komplex antagonisté a inhibitory   metabolismus   MeSH
• kosterní svaly enzymologie   MeSH
• krysa rodu Rattus MeSH
• malátdehydrogenasa účinky léků   metabolismus   MeSH
• membránový potenciál mitochondrií účinky léků   MeSH
• oniové sloučeniny farmakologie   MeSH
• potkani Wistar MeSH
• pyruvátdehydrogenasový komplex účinky léků   metabolismus   MeSH
• svalové mitochondrie účinky léků   enzymologie   MeSH
• tritylové sloučeniny farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• citrátsynthasa MeSH
• glutamátdehydrogenasa MeSH
• isocitrátdehydrogenasa MeSH
• ketoglutarátdehydrogenasový komplex MeSH
• malátdehydrogenasa MeSH
• oniové sloučeniny MeSH
• pyruvátdehydrogenasový komplex MeSH
• triphenylmethylphosphonium MeSH Prohlížeč
• tritylové sloučeniny MeSH