Yeast harbor several proteins with predicted glucanase activity that are potentially involved in cell wall remodeling during different processes, including mitosis. Here, we showed that 2 of these putative glucanases, Sun4p and Dse2p, co-localize to the yeast birth scar, dependently on presence of the third glucanase, Egt2p. The absence of these glucanases results in inefficient mother-daughter cell separation. The Sun4p, Dse2p and Egt2p localize to the daughter side of the bud neck, possibly forming a complex, and are involved in the separation of the virgin daughter from the mother cell during mitosis. The formation of properly assembled birth scars that delimitate cell wall area restricted in the next budding is dependent on the presence of Aim44p and its transcriptional regulator, Swi5p. AIM44 or SWI5 deletion caused the "budding within the birth scar" phenotype, together with altered localization of the birth scar proteins Sun4p and Dse2p, indicating the impairment of birth scar protein complexes.

• Klíčová slova
• SUN family of proteins, cell wall, glucanases, yeast birth scar,
• MeSH
• buněčná stěna metabolismus   MeSH
• fenotyp MeSH
• genový knockout MeSH
• glukosidasy metabolismus   MeSH
• Saccharomyces cerevisiae - proteiny metabolismus   MeSH
• Saccharomyces cerevisiae cytologie   metabolismus   MeSH
• transkripční faktory metabolismus   MeSH
• transport proteinů MeSH
• vazba proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glukosidasy MeSH
• Saccharomyces cerevisiae - proteiny MeSH
• Sun4 protein, S cerevisiae MeSH Prohlížeč
• transkripční faktory MeSH

Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis. Using a spectrum of different inhibitors, we showed that glycolysis is essential for the formation, and particularly, the survival of U cells. We also showed that β-1,3-glucans that are released from the cell walls of L cells are the most likely source of carbohydrates for U cells.

• Klíčová slova
• enzymatic assays, fermentation, metabolic differentiation, respiration, yeast colonies,
• MeSH
• beta-glukany metabolismus   MeSH
• buněčná stěna metabolismus   MeSH
• časové faktory MeSH
• fenotyp MeSH
• fermentace * účinky léků   MeSH
• genotyp MeSH
• glykolýza * účinky léků   MeSH
• inhibitory enzymů farmakologie   MeSH
• kultivační média chemie   metabolismus   MeSH
• mikrobiální viabilita MeSH
• mikrobiologické techniky metody   MeSH
• počet mikrobiálních kolonií MeSH
• Saccharomyces cerevisiae - proteiny genetika   metabolismus   MeSH
• Saccharomyces cerevisiae účinky léků   enzymologie   genetika   růst a vývoj   MeSH
• sériové pasážování MeSH
• substrátová specifita MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• beta-glukany MeSH
• inhibitory enzymů MeSH
• kultivační média MeSH
• Saccharomyces cerevisiae - proteiny MeSH