We have developed a robust solid-phase protocol which allowed the synthesis of chimeric oligonucleotides modified with phosphodiester and O-methylphosphonate linkages as well as their P-S and P-N variants. The novel O-methylphosphonate-derived modifications were obtained by oxidation, sulfurization, and amidation of the O-methyl-(H)-phosphinate internucleotide linkage introduced into the oligonucleotide chain by H-phosphonate chemistry using nucleoside-O-methyl-(H)-phosphinates as monomers. The H-phosphonate coupling followed by oxidation after each cycle enabled us to successfully combine H-phosphonate and phosphoramidite chemistries to synthesize diversely modified oligonucleotide strands.

• Klíčová slova
• H-phosphinate, H-phosphonate, oligonucleotide, phosphonamidate, phosphonate, phosphonothioate, phosphoramidate, phosphoramidite, phosphorothioate,
• MeSH
• amidy chemie   MeSH
• dimerizace MeSH
• fosfáty chemie   MeSH
• fosforothioátové oligonukleotidy chemická syntéza   MeSH
• kyseliny fosforečné chemie   MeSH
• molekulární struktura MeSH
• oligonukleotidy chemická syntéza   chemie   MeSH
• techniky syntézy na pevné fázi * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• amidy MeSH
• fosfáty MeSH
• fosforothioátové oligonukleotidy MeSH
• kyseliny fosforečné MeSH
• oligonukleotidy MeSH
• phosphoramidic acid MeSH Prohlížeč
• phosphorodithioic acid MeSH Prohlížeč