Protein-coated resorbable synthetic polymeric nanofibrous membranes are promising for the fabrication of advanced skin substitutes. We fabricated electrospun polylactic acid and poly(lactide-co-glycolic acid) nanofibrous membranes and coated them with fibrin or collagen I. Fibronectin was attached to a fibrin or collagen nanocoating, in order further to enhance the cell adhesion and spreading. Fibrin regularly formed a coating around individual nanofibers in the membranes, and also formed a thin noncontinuous nanofibrous mesh on top of the membranes. Collagen also coated most of the fibers of the membrane and randomly created a soft gel on the membrane surface. Fibronectin predominantly adsorbed onto a thin fibrin mesh or a collagen gel, and formed a thin nanofibrous structure. Fibrin nanocoating greatly improved the attachment, spreading, and proliferation of human dermal fibroblasts, whereas collagen nanocoating had a positive influence on the behavior of human HaCaT keratinocytes. In addition, fibrin stimulated the fibroblasts to synthesize fibronectin and to deposit it as an extracellular matrix. Fibrin coating also showed a tendency to improve the ultimate tensile strength of the nanofibrous membranes. Fibronectin attached to fibrin or to a collagen coating further enhanced the adhesion, spreading, and proliferation of both cell types.

• Klíčová slova
• collagen, fibrin, nanocoating, nanofibers, skin cells, skin-tissue engineering,
• MeSH
• buněčná adheze MeSH
• extracelulární matrix metabolismus   MeSH
• fibrin metabolismus   MeSH
• fibroblasty cytologie   metabolismus   MeSH
• fibronektiny metabolismus   MeSH
• keratinocyty cytologie   metabolismus   MeSH
• kolagen metabolismus   MeSH
• kultivované buňky MeSH
• lidé MeSH
• nanovlákna chemie   MeSH
• pevnost v tahu MeSH
• polymery chemie   MeSH
• proliferace buněk MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fibrin MeSH
• fibronektiny MeSH
• kolagen MeSH
• polymery MeSH

Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly(l-lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta1-integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering.

• Klíčová slova
• ascorbic acid, beta1-integrins, collagen I synthesis, electrospun nanofibers, fibrin, fibroblasts, nanocoating, nanomedicine, nanotechnology, skin tissue engineering,
• MeSH
• buněčná diferenciace MeSH
• elektrochemie metody   MeSH
• extracelulární matrix metabolismus   MeSH
• fibrin chemie   metabolismus   MeSH
• fibroblasty cytologie   metabolismus   MeSH
• fluorescenční protilátková technika MeSH
• imunoenzymatické techniky MeSH
• kolagen genetika   metabolismus   MeSH
• kultivované buňky MeSH
• kůže cytologie   metabolismus   MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• lidé MeSH
• messenger RNA genetika   MeSH
• nanovlákna chemie   MeSH
• polyestery chemie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• proliferace buněk MeSH
• regenerace fyziologie   MeSH
• tkáňové inženýrství metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• fibrin MeSH
• kolagen MeSH
• messenger RNA MeSH
• poly(lactide) MeSH Prohlížeč
• polyestery MeSH