Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm-oocyte adhesion/fusion and early implantation in the absence of sperm β1 integrin. Males and females with β1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm β1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin β1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern.

• Klíčová slova
• Stra8-Cre, fertilization, integrin beta1, sperm, ultrasound,
• MeSH
• antigeny CD29 * genetika   metabolismus   MeSH
• fertilizace MeSH
• integriny metabolismus   MeSH
• interakce spermie a vajíčka * MeSH
• myši MeSH
• oocyty metabolismus   MeSH
• sperma metabolismus   MeSH
• spermie metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny CD29 * MeSH
• integriny MeSH

The presence of laminin-1, collagen-IV, alpha6 and beta1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-1-, beta1-, and alpha6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.

• MeSH
• antigeny CD29 metabolismus   MeSH
• CD antigeny metabolismus   MeSH
• imunohistochemie MeSH
• integrin alfa6 MeSH
• integriny metabolismus   MeSH
• kolagen metabolismus   MeSH
• kryoterapie MeSH
• krysa rodu Rattus MeSH
• laminin metabolismus   MeSH
• nervus ischiadicus cytologie   metabolismus   transplantace   MeSH
• Schwannovy buňky cytologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD29 MeSH
• CD antigeny MeSH
• integrin alfa6 MeSH
• integriny MeSH
• kolagen MeSH
• laminin 1 MeSH Prohlížeč
• laminin MeSH

Bystander effects have been proposed as a third action pathway of ionising radiation besides direct and indirect effects. The purpose of the study was to investigate whether expression of interleukin-1alpha (IL-1alpha) and beta1-integrin is elevated in bystander cells as a marker for bystander effects in comparison with classical markers such as the clonogenic assay, apoptosis and the presence of micronuclei. The hybrid cell line E.A. hy.926 obtained by fusion of HUVEC cells with the epithelial cell line A 459 was irradiated with 0-5 Gy. Bystander effects were established via medium transfer at 45 min and 4 h after irradiation from irradiated to nonirradiated cell populations. In order to exclude effects of the irradiated medium itself, irradiated medium only was also used for transfer to nonirradiated cells. Then, cells were fixed at 1, 2, 6, and 24 h after irradiation or medium transport and IL-1alpha and beta1-integrin were detected and evaluated. A higher number of beta1-integrin-positive cells was observed in both irradiated and bystander cell populations than in the control group at 1 and 24 h after irradiation with 1 Gy or medium transfer. Significantly higher numbers of IL-1alpha-positive cells were found at 1, 2, and 6 h after irradiation with 1 Gy or medium transfer as well as at 2 and 6 h after irradiation with 5 Gy or medium transfer. Clonogenic survival decreased dependently on the dose in irradiated cells but did not show any significant difference between the bystander cell populations and sham-irradiated cells. The irradiated medium itself did not have any effect. It is concluded that beta1-integrin and IL-1alpha expression may serve as more sensitive markers of post-irradiation responses in bystander cell populations than the classical radiobiological markers. Moreover, overexpression of beta1-integrin and IL-1alpha may induce increased susceptibility to inflammation of bystander cells.

• MeSH
• analýza kolonii tvořících jednotek MeSH
• antigeny CD29 metabolismus   MeSH
• apoptóza MeSH
• biologické markery MeSH
• buněčné linie MeSH
• bystander efekt * MeSH
• interleukin-1 metabolismus   MeSH
• ionizující záření * MeSH
• kultivační média speciální MeSH
• lidé MeSH
• mikrojádra chromozomálně defektní metabolismus   MeSH
• pilotní projekty MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD29 MeSH
• biologické markery MeSH
• interleukin-1 MeSH
• kultivační média speciální MeSH

Lower respiratory tract infection due to Pseudomonas aeruginosa has become increasingly challenging, resulting in a worse morbidity and mortality. Airway remodeling is a common phenomenon in this process, to which epithelial-mesenchymal transition (EMT) may contribute as an important promoter. Previous studies showed that epithelium-specific integrin αvβ6-mediated EMT was involved in pulmonary fibrosis via transforming growth factor-β1 (TGF-β1) signaling, but whether integrin αvβ6 plays a role in the P. aeruginosa-associated airway remodeling remains unknown. BEAS-2B cells were incubated with lipopolysaccharide (LPS) from P. aeruginosa in the presence or the absence of integrin αvβ6-blocking antibodies. Morphologic changes were observed by an inverted microscopy. The EMT markers were detected using Western blotting and immunofluorescence. The activation of TGF-β1-Smad2/3 signaling pathway was assessed. Furthermore, matrix metalloproteinase (MMP)-2 and -9 in the medium were measured using ELISA. P. aeruginosa's LPS decreased the expression of the epithelial marker E-cadherin and promoted the mesenchymal markers, vimentin and α-smooth muscle actin in BEAS-2B cells. The expression of integrin αvβ6 was significantly increased during EMT process. Blocking integrin αvβ6 could attenuate P. aeruginosa's LPS-induced EMT markers' expression via TGF-β1-Smad2/3 signaling pathway. Furthermore, blocking integrin αvβ6 could prevent morphologic changes and oversecretion of MMP-2 and -9. Integrin αvβ6 mediates epithelial-mesenchymal transition in human bronchial epithelial cells induced by lipopolysaccharides of P. aeruginosa via TGF-β1-Smad2/3 signaling pathway and might be a promising therapeutic target for P. aeruginosa-associated airway remodeling.

• MeSH
• antigeny nádorové genetika   metabolismus   MeSH
• epitelo-mezenchymální tranzice * MeSH
• epitelové buňky cytologie   účinky léků   metabolismus   MeSH
• integriny genetika   metabolismus   MeSH
• lidé MeSH
• lipopolysacharidy metabolismus   MeSH
• matrixové metaloproteinasy genetika   metabolismus   MeSH
• protein Smad2 genetika   metabolismus   MeSH
• protein Smad3 genetika   metabolismus   MeSH
• pseudomonádové infekce genetika   metabolismus   mikrobiologie   patofyziologie   MeSH
• Pseudomonas aeruginosa metabolismus   MeSH
• signální transdukce MeSH
• transformující růstový faktor beta1 genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny nádorové MeSH
• integrin alphavbeta6 MeSH Prohlížeč
• integriny MeSH
• lipopolysacharidy MeSH
• matrixové metaloproteinasy MeSH
• protein Smad2 MeSH
• protein Smad3 MeSH
• SMAD2 protein, human MeSH Prohlížeč
• SMAD3 protein, human MeSH Prohlížeč
• transformující růstový faktor beta1 MeSH

Fibrin plays an important role during wound healing and skin regeneration. It is often applied in clinical practice for treatment of skin injuries or as a component of skin substitutes. We prepared electrospun nanofibrous membranes made from poly(l-lactide) modified with a thin fibrin nanocoating. Fibrin surrounded the individual fibers in the membrane and also formed a thin fibrous mesh on several places on the membrane surface. The cell-free fibrin nanocoating remained stable in the cell culture medium for 14 days and did not change its morphology. On membranes populated with human dermal fibroblasts, the rate of fibrin degradation correlated with the degree of cell proliferation. The cell spreading, mitochondrial activity, and cell population density were significantly higher on membranes coated with fibrin than on nonmodified membranes, and this cell performance was further improved by the addition of ascorbic acid in the cell culture medium. Similarly, fibrin stimulated the expression and synthesis of collagen I in human dermal fibroblasts, and this effect was further enhanced by ascorbic acid. The expression of beta1-integrins was also improved by fibrin, and on pure polylactide membranes, it was slightly enhanced by ascorbic acid. In addition, ascorbic acid promoted deposition of collagen I in the form of a fibrous extracellular matrix. Thus, the combination of nanofibrous membranes with a fibrin nanocoating and ascorbic acid seems to be particularly advantageous for skin tissue engineering.

• Klíčová slova
• ascorbic acid, beta1-integrins, collagen I synthesis, electrospun nanofibers, fibrin, fibroblasts, nanocoating, nanomedicine, nanotechnology, skin tissue engineering,
• MeSH
• buněčná diferenciace MeSH
• elektrochemie metody   MeSH
• extracelulární matrix metabolismus   MeSH
• fibrin chemie   metabolismus   MeSH
• fibroblasty cytologie   metabolismus   MeSH
• fluorescenční protilátková technika MeSH
• imunoenzymatické techniky MeSH
• kolagen genetika   metabolismus   MeSH
• kultivované buňky MeSH
• kůže cytologie   metabolismus   MeSH
• kvantitativní polymerázová řetězová reakce MeSH
• lidé MeSH
• messenger RNA genetika   MeSH
• nanovlákna chemie   MeSH
• polyestery chemie   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• proliferace buněk MeSH
• regenerace fyziologie   MeSH
• tkáňové inženýrství metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• fibrin MeSH
• kolagen MeSH
• messenger RNA MeSH
• poly(lactide) MeSH Prohlížeč
• polyestery MeSH

Chemotaxis of mast cells is one of the crucial steps in their development and function. Non-T cell activation linker (NTAL) is a transmembrane adaptor protein that inhibits the activation of mast cells and B cells in a phosphorylation-dependent manner. Here, we studied the role of NTAL in the migration of mouse mast cells stimulated by prostaglandin E2 (PGE2). Although PGE2 does not induce the tyrosine phosphorylation of NTAL, unlike IgE immune complex antigens, we found that loss of NTAL increased the chemotaxis of mast cells toward PGE2 Stimulation of mast cells that lacked NTAL with PGE2 enhanced the phosphorylation of AKT and the production of phosphatidylinositol 3,4,5-trisphosphate. In resting NTAL-deficient mast cells, phosphorylation of an inhibitory threonine in ERM family proteins accompanied increased activation of β1-containing integrins, which are features often associated with increased invasiveness in tumors. Rescue experiments indicated that only full-length, wild-type NTAL restored the chemotaxis of NTAL-deficient cells toward PGE2 Together, these data suggest that NTAL is a key inhibitor of mast cell chemotaxis toward PGE2, which may act through the RHOA/ERM/β1-integrin and PI3K/AKT axes.

• MeSH
• adaptorové proteiny signální transdukční metabolismus   MeSH
• aktiny metabolismus   MeSH
• antigeny CD29 metabolismus   MeSH
• bodová mutace MeSH
• chemotaxe * MeSH
• cholesterol metabolismus   MeSH
• dinoproston metabolismus   MeSH
• fosfatidylinositol-3-kinasy metabolismus   MeSH
• fosforylace MeSH
• integriny metabolismus   MeSH
• mastocyty metabolismus   MeSH
• membránové proteiny metabolismus   MeSH
• myši MeSH
• proteinové domény MeSH
• proteiny metabolismus   MeSH
• signální transdukce MeSH
• threonin chemie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adaptorové proteiny signální transdukční MeSH
• aktiny MeSH
• antigeny CD29 MeSH
• cholesterol MeSH
• dinoproston MeSH
• integriny MeSH
• LAT2 protein, mouse MeSH Prohlížeč
• membránové proteiny MeSH
• proteiny MeSH
• threonin MeSH

A basal layer of squamous epithelia such as epidermis contains stem cells, transit amplifying cells as well as postmitotic differentiating cells. A detailed knowledge of the transition among these cell types in the course of epidermal renewal is important. It would help in better understanding of many pathological processes, including cancer, and in employment of epidermal cells for therapeutic purposes. In this study we analyzed the possible role of Dolichos biflorus agglutinin (DBA)-reactive alpha-N-acetylgalactosamine glycosylation in behavior of the human epidermal basal cells under in vivo and in vitro conditions. The data received from porcine epidermis were also included. Part of basal cells was positive for DBA-binding sites and these cells exhibited a lower presence of beta1 integrin in their basal surface connected to the basement membrane. The perinuclear Golgi-like accumulation of beta1 integrin was observed in some cultured keratinocytes. The co-localization of integrin with DBA-binding sites and 58 kDa protein suggests that alpha-N-acetylgalactosamine glycosylation could be related to beta1 integrin retention in the endoplasmatic reticulum Golgi intermediate compartment (ERGIC) at the beginning of the secretory pathway. The lack of anchorage in culture elevated the number of DBA-binding site positive cells without significant influence on cell growth when cells isolated directly from epidermis were employed in study. Some role of DBA-reactive glycoligand expressions in a suprabasal movement of differentiated basal cells can be hypothesized.

• MeSH
• acetylgalaktosamin metabolismus   MeSH
• antigeny CD29 analýza   MeSH
• buněčná adheze MeSH
• buněčná diferenciace MeSH
• dospělí MeSH
• druhová specificita MeSH
• epidermální buňky MeSH
• glykosylace MeSH
• keratinocyty chemie   cytologie   MeSH
• lidé MeSH
• plod MeSH
• pohlavní dimorfismus MeSH
• prasata MeSH
• rostlinné lektiny metabolismus   MeSH
• vazebná místa MeSH
• vývojová regulace genové exprese * MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetylgalaktosamin MeSH
• antigeny CD29 MeSH
• dolichos biflorus agglutinin MeSH Prohlížeč
• rostlinné lektiny MeSH

Keratin 19 and nuclear reactivity to an endogenous lectin, galectin-1, represent a potential marker of epidermal stem cells. We detected expression of keratin 19 and nuclear binding sites for galectin-1 in adult cells migrating from the hair follicle, where cells expressing keratin 19 are located in the bulge region. The results were compared with the expression of both markers in cells adhering from suspension prepared from the interfollicular epidermis without keratin-19-positive cells and with nuclear binding sites for galectin-1. The results were compared with data from basal cell carcinomas. All cells were analyzed concerning size, as it is known that cell diameter influences the clonogenic potential of keratinocytes. The major result of this study is the observation of transient expression of keratin 19 and nuclear galectin-1 binding sites in originally negative interfollicular epidermal cells induced by adhesion. These cells were very small in size, similar to basal cells of the interfollicular epidermis or the bulge region of the hair follicle. The influence of the suspension regimen on beta1-integrin expression, cell diameter and growth was also monitored. A population of cells highly positive for beta1 integrin of the same diameter as keratin-19-positive cells insensitive to induction of terminal differentiation by lack of anchorage was characterized. Cells of the same size were also observed in the keratin-19-positive cells of basal cell carcinomas. In conclusion, the expression of poor levels of differentiation induced by cell adhesion is transient. Also, keratin 19 expression should not be exclusively regarded as a marker of stem cell activity.

• MeSH
• antigeny CD29 analýza   MeSH
• bazocelulární karcinom metabolismus   patologie   MeSH
• buněčná adheze MeSH
• buněčné kultury MeSH
• časové faktory MeSH
• epidermální buňky MeSH
• epidermis chemie   MeSH
• galektin 1 analýza   MeSH
• galektin 3 analýza   MeSH
• keratin-10 MeSH
• keratin-20 MeSH
• keratinocyty chemie   cytologie   MeSH
• keratiny analýza   MeSH
• kultivované buňky MeSH
• lidé MeSH
• nádory kůže metabolismus   patologie   MeSH
• pohyb buněk MeSH
• proteiny intermediálních filament analýza   MeSH
• vlasový folikul chemie   cytologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny CD29 MeSH
• galektin 1 MeSH
• galektin 3 MeSH
• keratin-10 MeSH
• keratin-20 MeSH
• keratiny MeSH
• KRT10 protein, human MeSH Prohlížeč
• KRT20 protein, human MeSH Prohlížeč
• proteiny intermediálních filament MeSH

We have found that pretreatment of a human oral mucosal epithelial cell line KB with natural human interferon-alpha (IFN-alpha) augments the expression of intercellular adhesion molecule-1 (ICAM-1), and the complex of CD29 and CD49b, which is known as very late antigen (VLA)-2, in a dose-dependent manner, whereas the expression of other adhesion molecules such as CD44 leukocyte function associated antigen-3 (LFA-3), VLA-4 and endothelial leukocyte adhesion molecule-1 (ELAM-1) did not show any significant changes under the same conditions. The pretreatment of KB cells with IFN-alpha also induces the adhesion of these cells to the human leukemia T cell line MOLT-16 and to peripheral T lymphocytes in a dose-dependent manner. However, the adhesion of the above-mentioned T cells to KB cells was not inhibited by specific antibodies against ICAM-1, CD29, and CD49b. The results thus show that adhesion molecules other than ICAM-1, CD29, AND CD49b are responsible for the induced adhesion between T cells and IFN-alpha-pretreated KB cells.

• MeSH
• antigeny CD29 biosyntéza   MeSH
• buněčná adheze MeSH
• buněčné linie MeSH
• CD antigeny biosyntéza   MeSH
• epitel metabolismus   MeSH
• epitelové buňky MeSH
• integrin alfa2 MeSH
• interferon alfa farmakologie   MeSH
• lidé MeSH
• mezibuněčná adhezivní molekula-1 biosyntéza   MeSH
• T-lymfocyty metabolismus   patologie   MeSH
• ústní sliznice cytologie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny CD29 MeSH
• CD antigeny MeSH
• integrin alfa2 MeSH
• interferon alfa MeSH
• mezibuněčná adhezivní molekula-1 MeSH

Suberoylanilide hydroxamic acid (SAHA) is an inhibitor of histone deacetylases (HDACs) which is being introduced into clinic for the treatment of hematological diseases. We studied the effect of this compound on six human hematopoietic cell lines (JURL-MK1, K562, CML-T1, Karpas-299, HL-60, and ML-2) as well as on normal human lymphocytes and on leukemic primary cells. SAHA induced dose-dependent and cell type-dependent cell death which displayed apoptotic features (caspase-3 activation and apoptotic DNA fragmentation) in most cell types including the normal lymphocytes. At subtoxic concentrations (0.5-1 microM), SAHA increased the cell adhesivity to fibronectin (FN) in all leukemia/lymphoma-derived cell lines but not in normal lymphocytes. This increase was accompanied by an enhanced expression of integrin beta1 and paxillin, an essential constituent of focal adhesion complexes, both at the protein and mRNA level. On the other hand, the inhibition of ROCK protein, an important regulator of cytoskeleton structure, had no consistent effect on SAHA-induced increase in the cell adhesivity. The promotion of cell adhesivity to FN seems to be specific for SAHA as we observed no such effects with other HDAC inhibitors (trichostatin A and sodium butyrate).

• MeSH
• antigeny CD29 metabolismus   MeSH
• apoptóza účinky léků   MeSH
• buněčná adheze účinky léků   MeSH
• fibronektiny metabolismus   MeSH
• kyseliny hydroxamové farmakologie   MeSH
• leukemie metabolismus   MeSH
• lidé MeSH
• lymfocyty účinky léků   metabolismus   MeSH
• nádorové buněčné linie MeSH
• paxilin metabolismus   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• protinádorové látky farmakologie   MeSH
• průtoková cytometrie MeSH
• separace buněk MeSH
• vorinostat MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD29 MeSH
• fibronektiny MeSH
• kyseliny hydroxamové MeSH
• paxilin MeSH
• protinádorové látky MeSH
• vorinostat MeSH