Glyphosate (N-(phosphonomethyl)glycine) is an active substance of many herbicides. According to literature studies, glyphosate residues and their metabolites have been commonly detected in surface waters and toxicological reports confirmed negative effects on living organisms. In this study, the acute embryo toxicity of glyphosate into two different fish species-common carp (Cyprinus carpio) and zebrafish (Danio rerio)-was investigated. Lethal endpoints, development disorder, and, in addition, other sublethal endpoints such as hatching rate, formation of somites, and development of eyes, spontaneous movement, heartbeat/blood circulation, pigmentation, and edema were recorded to indicate the mode of action of the toxic compound. Hatching retardation (p < 0.05) was observed in experimental groups of common carp exposed to glyphosate with significant statistical difference especially at the highest concentration after 72, 96, and 120 hpf. The significantly highest cumulative mortality at concentration of 50 mg/l was observed. In contrast, hatching stimulation was observed in embryos of zebrafish exposed to the highest concentration of glyphosate. The significantly highest cumulative mortality for zebrafish was observed only at concentration of 50 mg/l. Based on our results, early life stages of common carp are more sensitive in comparison to zebrafish to the toxic action of glyphosate.

• Klíčová slova
• Embryo toxicity tests, Fish, Glyphosate, Hatching, Herbicide, Malformation, Mortality,
• MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• dánio pruhované růst a vývoj   MeSH
• druhová specificita MeSH
• embryo nesavčí účinky léků   MeSH
• embryonální vývoj účinky léků   MeSH
• glycin analogy a deriváty   toxicita   MeSH
• glyfosát MeSH
• herbicidy toxicita   MeSH
• kapři růst a vývoj   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• glycin MeSH
• herbicidy MeSH

The sensitivity of early life stages of common carp (Cyprinus carpio L.) to chronic exposure to single and combined environmental concentrations of the triazine metabolites terbuthylazine 2-hydroxy, terbuthylazine-desethyl and atrazine 2-hydroxy was evaluated under laboratory conditions. Their effects were assessed on lipid peroxidation, antioxidant enzymes (total superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, reduced glutathione), mortality, growth, development and histology. Single metabolites (terbuthylazine 2-hydroxy-0.73 μg/L; terbuthylazine-desethyl-1.80 μg/L; atrazine 2-hydroxy-0.66 μg/L) and combinations were not associated with negative effects on hatching, behaviour, embryo viability, growth or early ontogeny. Carp exposed to terbuthylazine-desethyl at 1.80 μg/L showed significantly lower total superoxide dismutase and glutathione reductase activity compared with the control group. Liver histology revealed diffused steatosis associated with the presence of lipid inclusions in hepatic cells in groups exposed to terbuthylazine-desethyl, atrazine 2-hydroxy and the tested combination of metabolites.

• Klíčová slova
• Antioxidant enzymes, Atrazine 2-hydroxy, Fish, Terbuthylazine 2-hydroxy, Terbuthylazine-desethyl,
• MeSH
• antioxidancia metabolismus   MeSH
• atrazin metabolismus   toxicita   MeSH
• chemické látky znečišťující vodu metabolismus   toxicita   MeSH
• embryo nesavčí účinky léků   enzymologie   metabolismus   MeSH
• glutathion metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• herbicidy metabolismus   toxicita   MeSH
• játra účinky léků   embryologie   patologie   MeSH
• kapři embryologie   metabolismus   MeSH
• katalasa metabolismus   MeSH
• látky reagující s kyselinou thiobarbiturovou metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• peroxidace lipidů účinky léků   MeSH
• superoxiddismutasa metabolismus   MeSH
• triaziny metabolismus   toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• atrazin MeSH
• chemické látky znečišťující vodu MeSH
• glutathion MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• herbicidy MeSH
• katalasa MeSH
• látky reagující s kyselinou thiobarbiturovou MeSH
• superoxiddismutasa MeSH
• terbutylazine MeSH Prohlížeč
• triaziny MeSH