Nejvíce citovaný článek - PubMed ID 26289098
Xenobiotic-metabolizing enzymes in plants and their role in uptake and biotransformation of veterinary drugs in the environment
In recent years, there has been increasing concern over the environmental risks of the so called "Emerging pollutants (EPs)" that are defined as synthetic or naturally occurring chemicals that are not commonly monitored in the environment but which have the potential to enter the environment and cause adverse ecological and (or) human health effects [...].
Albendazole (ABZ), a widely used anthelmintic drug, enters the environment mainly via livestock excrements. To evaluate the environmental impact of ABZ, the knowledge of its uptake, effects and metabolism in all non-target organisms, including plants, is essential. The present study was designed to identify the metabolic pathway of ABZ and to test potential ABZ phytotoxicity in fodder plant alfalfa, with seeds and in vitro regenerants used for these purposes. Alfalfa was chosen, as it may meet manure from ABZ-treated animals in pastures and fields. Alfalfa is often used as a feed of livestock, which might already be infected with helminths. The obtained results showed that ABZ did not inhibit alfalfa seed germination and germ growth, but evoked stress and a toxic effect in alfalfa regenerants. Alfalfa regenerants were able to uptake ABZ and transform it into 21 metabolites. UHPLC-MS/MS analysis revealed three new ABZ metabolites that have not been described yet. The discovery of the parent compound ABZ together with the anthelmintically active and instable metabolites in alfalfa leaves shows that the contact of fodder plants with ABZ-containing manure might represent not only a danger for herbivorous invertebrates, but also may cause the development of ABZ resistance in helminths.
- Klíčová slova
- UHPLC-MS/MS, anthelmintics, drug metabolism, drug phytotoxicity, drugs in the environment,
- MeSH
- albendazol farmakologie MeSH
- anthelmintika farmakologie MeSH
- klíčení MeSH
- krmivo pro zvířata MeSH
- Medicago sativa účinky léků růst a vývoj metabolismus MeSH
- metabolom * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- albendazol MeSH
- anthelmintika MeSH