This review focuses on the development and applications of organic polymer monoliths, with special attention to the literature published in 2021. The latest protocols in the preparation of polymer monoliths are discussed. In particular, tailored surface modification using nanomaterials, the development of chiral stationary phases and development of stationary phases for capillary electrochromatography are reviewed. Furthermore, the optimization of pore forming solvents composition is also discussed. Finally, the use of monolithic stationary phases in sample treatment using solid-phase extraction and enrichment methods, molecularly imprinted polymers and enzymatic reactors is mentioned.

• Klíčová slova
• column technology, liquid chromatography, monolith, sample treatment, surface modification,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

This review highlights the current trends and the most recent advances in the field of preparation and application of organic polymer-based monolithic materials and covers literature published in 2020. A short historical background is provided and protocols to anchor monoliths covalently to the wall of the column/separation device are discussed. Furthermore, advances in tuning the macroporous structure and establishing its link to separation performance are conferred. Finally, method development and key applications using novel monolithic columns are discussed.

• Klíčová slova
• bioanalysis, column technology, review, stationary phase,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH