Enterococci are widespread bacteria forming the third largest genus among lactic acid bacteria. Some possess probiotic properties or they can produce beneficial proteinaceous antimicrobial substances called enterocins. On the other hand, some enterococci produce biogenic amines (BAs), so this study is focused on the sensitivity to enterocins of biogenic amine-producing faecal enterococci from ostriches and pheasants. Altogether, 60 enterococci isolated from faeces of ostriches and pheasants were tested for production of BAs. This target of the identified enterococci involved 46 strains selected from 140 ostriches and 17 from 60 pheasants involving the species Enterococcus hirae, E. faecium, E. faecalis, and E. mundtii. Although BAs histamine, cadaverine, putrescine, and tryptamine were not detected in the enterococci tested, in general high BA production by the tested enterococci was noted. The species E. hirae formed the majority of the enterococcal strains from ostrichs faeces (34 strains). High production of tyramine (TYM) was measured with an average amount of 958.16 ± 28.18 mg/ml. Among the enterococci from pheasants, the highest was production of TYM compared to phenylethylamine, spermidine, and spermine. Enterococci featured high BA production; however, they were sensitive to seven enterocins with inhibition activity ranging from 100 up to 25,600 AU/ml.

• Klíčová slova
• Biogenic amines, Enterocins, Enterococci, Poultry, Production, Sensitivity,
• MeSH
• biogenní aminy biosyntéza   MeSH
• Enterococcus klasifikace   izolace a purifikace   MeSH
• feces chemie   mikrobiologie   MeSH
• fenethylaminy metabolismus   MeSH
• Galliformes mikrobiologie   MeSH
• přemostěné cyklické sloučeniny metabolismus   MeSH
• spermidin biosyntéza   MeSH
• spermin biosyntéza   MeSH
• Struthioniformes mikrobiologie   MeSH
• tyramin biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biogenní aminy MeSH
• enterocin MeSH Prohlížeč
• fenethylaminy MeSH
• přemostěné cyklické sloučeniny MeSH
• spermidin MeSH
• spermin MeSH
• tyramin MeSH

In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococci from ostriches and pheasants and their sensitivity to lantibiotic bacteriocin gallidermin. Gallidermin belongs in a group of polycyclic proteinaceous antimicrobial substances. Thirty-six faecal staphylococci (24 strains from 140 ostriches, 12 from 60 pheasants) comprising different species were tested. Staphylococci from ostriches and pheasants did not produce tryptamine-TRYP, putrescine-PUT, cadaverine-CAD or histamine-HIS. Production of tyramine-TYM, phenylethylamine-PEA was high or very high (100-1000 mg/L). Production of spermine-SPM and spermidine-SPD by staphylococci was very low or low although in the case of staphylococci from pheasants medium production of SPM was found. Because of the risk posed by BAs for consumers, the control of BA-producing bacteria is important from the points of view not only of safety assessment of food-producing animals but also of human health safety. The sensitivity to gallidermin in biogenic amine-producing staphylococci from ostriches and pheasants detected here is the most promising indication for further application of gallidermin for veterinary purposes. The novelty of our study lies in testing the ability of faecal staphylococci from ostriches and pheasants to produce BAs and in their treatment with gallidermin which has so far not been tested in this way.

• Klíčová slova
• Biogenic amines, Gallidermin, Ostriches, Pheasants, Sensitivity, Staphylococci,
• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriociny farmakologie   MeSH
• biogenní aminy metabolismus   MeSH
• feces mikrobiologie   MeSH
• Galliformes mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• peptidy farmakologie   MeSH
• Staphylococcus účinky léků   izolace a purifikace   metabolismus   MeSH
• Struthioniformes mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriociny MeSH
• biogenní aminy MeSH
• gallidermin MeSH Prohlížeč
• peptidy MeSH