Silver nanoparticles (Ag NPs) significantly enhance the antibacterial activity of antibiotics and even restore their effect against resistant strains, making them a promising option for overcoming bacterial resistance to antibiotics. However, the exact mechanism of their synergistic effect with antibiotics at the cellular level has not been elucidated. In this work, we synthesised rhodamine-labelled Ag NPs and described, for the first time, the multi-level non-specific mechanism of the synergistic antibacterial effect of fluorescently labelled Ag NPs and a fluorescent vancomycin conjugate against vancomycin-resistant enterococci using high-resolution fluorescence microscopy. The multi-level mechanism of the synergistic effect of Ag NPs and vancomycin is mainly based on the disruption of the strength and integrity of the cell wall, which becomes unstable, loses strength and subsequently disintegrates due to the oxidative stress caused by Ag NPs and the residual effect of vancomycin. In addition, Ag NPs penetrate the bacterial cell and deform the bacterial DNA, which also significantly increases the synergistic antibacterial effect. This work represents an advance in understanding the mechanism of synergistic effect of Ag NPs with antibiotics against resistant bacteria, an important finding for a potential approach to effectively combat the unsolved problem of increasing resistance of pathogenic bacteria to traditional antibiotics.
- MeSH
- antibakteriální látky * farmakologie chemie chemická syntéza MeSH
- Enterococcus * účinky léků MeSH
- fluorescenční mikroskopie MeSH
- kovové nanočástice * chemie MeSH
- mikrobiální testy citlivosti MeSH
- stříbro * chemie farmakologie MeSH
- synergismus léků MeSH
- vankomycin * farmakologie chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- stříbro * MeSH
- vankomycin * MeSH
BACKGROUND: Since the incidence of vancomycin-resistant enterococci (VRE) is increasing and treatment options remain limited, we aimed to investigate the epidemiology of vancomycin- and tigecycline-resistant enterococci in a university hospital using whole genome sequencing (WGS). METHODS: Between April and December 2021, 102 VRE isolates were collected from a single tertiary care hospital in the Czech Republic. Forty selected isolates underwent antimicrobial susceptibility testing and WGS (Illumina short reads and long reads with MinION in selected isolates). RESULTS: All Enterococcus faecium isolates were resistant to ampicillin, carrying the PBP5_Met485Ala, PBP5_Glu629Val, and fluoroquinolones carrying the GyrA_Ser83Ile and ParC_Ser80Ile substitutions. The vanA operon was found on pELF2-like plasmids and plasmids carrying rep17 and/or rep18b genes. The novel Tn1546 structural variants were identified in vanA-carrying isolates. The vanB operon was located on the chromosome within a Tn1549 structural variant. Linezolid resistance was detected in one isolate carrying the 23S rDNA_G2576T substitution. Twenty-two isolates were resistant to tigecycline (tet(L), tet(M) and rpsJ_del 155-166 or RpsJ_Lys57Arg). Discrepancies between phenotypic and genotypic resistance profiles were observed for daptomycin (RpoB_Ser491Phe), trimethoprim/sulfamethoxazole (dfrG gene), nitrofurantoin (NmrA_Gln48Lys substitution without the EF0404 and EF0648 genes) and tetracycline (truncated TetM). The two multilocus sequence typing (MLST) schemes identified different numbers of STs: 5 STs, with ST117 as the predominant one (n = 32, 80%), versus 10 STs, with ST138 (27.5%), ST136 (25%), and ST1067 (20%) being the most frequent, respectively. The whole genome MLST revealed clonal clustering (0-7 allele differences) among isolates of the same ST. When comparing ST117 isolates from our study with 2,204 ST117 isolates from 15 countries, only one Czech isolate clustered closely with strains from Germany and the Netherlands, differing by just 16 alleles. CONCLUSIONS: The spread of E. faecium isolates ST117 resistant to vancomycin and tigecycline was identified. The discrepancies between resistance genotypes and phenotypes highlight the importance of combining molecular and phenotypic surveillance in antimicrobial resistance monitoring.
- Klíčová slova
- Daptomycin, Long-read sequencing, Plasmids, Structural variants, Whole-genome sequencing, antimicrobial resistance,
- MeSH
- antibakteriální látky * farmakologie MeSH
- bakteriální proteiny genetika MeSH
- Enterococcus faecium * genetika účinky léků izolace a purifikace klasifikace MeSH
- enterokoky rezistentní vůči vankomycinu * genetika účinky léků izolace a purifikace MeSH
- genom bakteriální MeSH
- grampozitivní bakteriální infekce * mikrobiologie epidemiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence genetika MeSH
- multilokusová sekvenční typizace MeSH
- rezistence na vankomycin genetika MeSH
- sekvenování celého genomu MeSH
- tigecyklin * farmakologie MeSH
- vankomycin * farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Názvy látek
- antibakteriální látky * MeSH
- bakteriální proteiny MeSH
- tigecyklin * MeSH
- vankomycin * MeSH
BACKGROUND AND OBJECTIVE: Microbial selenium (Se) supplementation is an essential area of biotechnological research due to differences in the bioavailability and toxicity of different forms of selenium. To date, research has focused mainly on the use of selenized yeast. However, in recent years, scientific interest has also increased in other microorganisms, such as lactic acid bacteria (LAB), which have several unique properties that can affect the quality and bioavailability of selenium. LAB, unlike yeast, can also act as probiotics, which may bring additional health benefits related to improving the intestinal microbiota and supporting the health of the gastrointestinal tract. METHODS: This study investigates the in vitro bioaccessibility and bioavailability of Se from two lactic acid bacterial strains, Streptococcus thermophilus CCDM 144 and Enterococcus faecium CCDM 922 A. We evaluated Se accumulation, speciation, and stability during simulated gastrointestinal digestion and Se permeation through a Caco-2 cell monolayer model. RESULTS: Both strains accumulated Se, metabolizing it predominantly into selenium nanoparticles (SeNPs, 64-77 % of total Se), with only a minor fraction (<5 % of total Se) of organic Se species. Experiments revealed that while organic Se species had high bioavailability (up to 90 %), their bioaccessibility during digestion was very low (<0.1 % of total Se). In contrast, SeNPs showed high bioaccessibility (∼90 %) and moderate transport efficiency through the intestinal model (16-19 % after 4 hours). CONCLUSION: These results highlight the potential of SeNPs produced by lactic acid bacteria as a bioaccessible form of Se for dietary supplementation. Further research is required to explore the behavior of SeNPs within the human body to fully understand how they can be used safely and effectively in nutrition or other applications.
- Klíčová slova
- Caco-2 cells, Enterococcus, Se species stability, Streptococcus, selenium nanoparticles,
- MeSH
- biologická dostupnost MeSH
- biologické modely * MeSH
- Caco-2 buňky MeSH
- Enterococcus faecium * metabolismus MeSH
- funkce střevní bariéry MeSH
- Lactobacillales * metabolismus MeSH
- lidé MeSH
- permeabilita MeSH
- selen * metabolismus farmakokinetika MeSH
- Streptococcus thermophilus * metabolismus MeSH
- trávení * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- selen * MeSH
Antimicrobial resistance remains a global issue, hindering the control of bacterial infections. This study examined the antimicrobial properties of 2,3-N,N-diphenyl quinoxaline derivatives against Gram-positive, Gram-negative, and Mycobacterium species. Two quinoxaline derivatives (compounds 25 and 31) exhibited significant activity against most strains of Staphylococcus aureus, Enterococcus faecium, and Enterococcus faecalis tested, with MIC values ranging from 0.25 to 1 mg/L. These compounds also showed effective antibacterial activity against methicillin-resistant S. aureus (MRSA) and vancomycin-resistant E. faecium/E. faecalis (VRE) strains. They demonstrated comparable or superior activity to four current antibiotics (vancomycin, teicoplanin, daptomycin, and linezolid) against a wide range of clinically relevant isolates. Additionally, they were more effective in preventing S. aureus and E. faecalis biofilm formation compared to several other antibiotics. In summary, these two quinoxaline derivatives have potential as new antibacterial agents.
- Klíčová slova
- Enterococcus faecalis, Enterococcus faecium, MRSA, Staphylococcus aureus, VRE, biofilm, quinoxaline,
- MeSH
- antibakteriální látky * farmakologie MeSH
- biofilmy * účinky léků růst a vývoj MeSH
- chinoxaliny * farmakologie MeSH
- Enterococcus faecalis * účinky léků MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus * účinky léků MeSH
- mikrobiální testy citlivosti * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- chinoxaliny * MeSH
An in-depth investigation was conducted on a promising composite material (BiVO4/TiO2), focusing on its potential toxicity, photoinduced catalytic properties, as well as its antibiofilm and antimicrobial functionalities. The preparation process involved the synthesis of 2D TiO2 using the lyophilization method, which was subsequently functionalized with sphere-like BiVO4 through wet impregnation. Finally, we developed BiVO4/TiO2 S-scheme heterojunctions which can greatly promote the separation of electron-hole pairs to achieve high photocatalytic performance. The evaluation of concentration- and time-dependent viability inhibition was performed on human lung carcinoma epithelial A549 cells. This assessment included the estimation of glutathione levels and mitochondrial dehydrogenase activity. Significantly, the BiVO4/TiO2 composite demonstrated minimal toxicity towards A549 cells. Impressively, the BiVO4/TiO2 composite exhibited notable photocatalytic performance in the degradation of rhodamine B (k=0.135 min-1) and phenol (k=0.016 min-1). In terms of photoinduced antimicrobial performance, the composite effectively inactivated both gram-negative E. coli and gram-positive E. faecalis bacteria upon 60 minutes of UV-A light exposure, resulting in a significant log 6 (log 10 CFU/mL) reduction in bacterial count. In addition, a 49 % reduction of E. faecalis biofilm was observed. These promising results can be attributed to the unique 2D morphology of TiO2 modified by sphere-like BiVO4, leading to an increased generation of (intracellular) hydroxyl radicals, which plays a crucial role in the treatments of both organic pollutants and bacteria. This research has significant potential for various applications, particularly in addressing environmental contamination and microbial infections.
- Klíčová slova
- 2D TiO2, antibiofilm, antimicrobial, cytotoxicity, photocatalysis, sphere-like BiVO4,
- MeSH
- antibakteriální látky * chemie farmakologie MeSH
- biofilmy účinky léků MeSH
- bismut * chemie MeSH
- buňky A549 MeSH
- Enterococcus faecalis účinky léků MeSH
- Escherichia coli * účinky léků MeSH
- fotochemické procesy * MeSH
- fotolýza MeSH
- katalýza MeSH
- lidé MeSH
- nanostruktury chemie MeSH
- rhodaminy chemie MeSH
- titan * chemie MeSH
- vanadáty * chemie farmakologie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- bismut * MeSH
- bismuth vanadium tetraoxide MeSH Prohlížeč
- rhodamine B MeSH Prohlížeč
- rhodaminy MeSH
- titan * MeSH
- titanium dioxide MeSH Prohlížeč
- vanadáty * MeSH
A series of new unique acetylene derivatives of 8-hydroxy- and 8-methoxyquinoline- 5-sulfonamide 3a-f and 6a-f were prepared by reactions of 8-hydroxy- and 8-methoxyquinoline- 5-sulfonyl chlorides with acetylene derivatives of amine. A series of new hybrid systems containing quinoline and 1,2,3-triazole systems 7a-h were obtained by reactions of acetylene derivatives of quinoline-5-sulfonamide 6a-d with organic azides. The structures of the obtained compounds were confirmed by 1H and 13C NMR spectroscopy and HR-MS spectrometry. The obtained quinoline derivatives 3a-f and 6a-f and 1,2,3-triazole derivatives 7a-h were tested for their anticancer and antimicrobial activity. Human amelanotic melanoma cells (C-32), human breast adenocarcinoma cells (MDA-MB-231), and human lung adenocarcinoma cells (A549) were selected as tested cancer lines, while cytotoxicity was investigated on normal human dermal fibroblasts (HFF-1). All the compounds were also tested against reference strains Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 and representatives of multidrug-resistant clinical isolates of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant E. faecalis. Only the acetylene derivatives of 8-hydroxyquinoline-5-sulfonamide 3a-f were shown to be biologically active, and 8-hydroxy-N-methyl-N-(prop-2-yn-1-yl)quinoline-5-sulfonamide (3c) showed the highest activity against all three cancer lines and MRSA isolates. Its efficacies were comparable to those of cisplatin/doxorubicin and oxacillin/ciprofloxacin. In the non-cancer HFF-1 line, the compound showed no toxicity up to an IC50 of 100 µM. In additional tests, compound 3c decreased the expression of H3, increased the transcriptional activity of cell cycle regulators (P53 and P21 proteins), and altered the expression of BCL-2 and BAX genes in all cancer lines. The unsubstituted phenolic group at position 8 of the quinoline is the key structural fragment necessary for biological activity.
- Klíčová slova
- 1,2,3-triazole, 8-hydroxyquinoline, acetylene derivatives, antibacterial activity, anticancer activity, cytotoxicity, synthesis,
- MeSH
- antibakteriální látky * farmakologie chemie chemická syntéza MeSH
- chinoliny * chemie farmakologie chemická syntéza MeSH
- Enterococcus faecalis účinky léků MeSH
- lidé MeSH
- mikrobiální testy citlivosti * MeSH
- molekulární struktura MeSH
- nádorové buněčné linie MeSH
- protinádorové látky * farmakologie chemie chemická syntéza MeSH
- racionální návrh léčiv MeSH
- Staphylococcus aureus účinky léků MeSH
- sulfonamidy * farmakologie chemie chemická syntéza MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- chinoliny * MeSH
- protinádorové látky * MeSH
- sulfonamidy * MeSH
OBJECTIVE: Feed additives have attracted increased attention in aquaculture due to their ability to modulate fish gut microbiota, resulting in improved fish growth and immunity. This study assessed the effects of two synbiotics in Japanese Eel Anguilla japonica: Bacillus subtilis with mannooligosaccharides (MOS) and Enterococcus faecium with fructooligosaccharides (FOS). METHODS: Six diets, including a control (CON) diet, oxytetracycline (OTC) diet, and four synbiotic diets (B. subtilis at 1 × 106 or 1 × 107 colony-forming units [CFU]/g with MOS at 5 g/kg [BS6MO and BS7MO; collectively, BSMOS diets] and E. faecium at 1 × 106 or 1 × 107 CFU/g with FOS at 5 g/kg [EF6FO and EF7FO; collectively, EFFOS diets]), were fed to triplicate groups of 20 fish (average weight ± SD = 6.00 ± 0.07 g) for 8 weeks. RESULT: Fish fed the BSMOS diets showed significantly higher weight gain, specific growth rate (SGR), and feed efficiency compared to fish fed the CON and OTC diets, but the values were not significantly different from those of fish fed the EFFOS diets. Weight gain and SGR of fish that were given EFFOS diets were not significantly different from those of fish fed all other diets. Fish fed the OTC diet showed a higher mean aspartate aminotransferase level, although the difference was not statistically significant. The myeloperoxidase activity of fish fed the BS7MO diet was significantly higher than those of fish receiving all other diets, and the superoxide dismutase activity of fish fed the BS7MO diet was also significantly higher than that of fish fed the EF7FO diet. Overall, the BSMOS synbiotic diets were significantly more effective than the CON diet in enhancing fish survival against a Vibrio anguillarum challenge. CONCLUSION: Our findings suggest that synbiotics can be a preferable alternative to antibiotics in aquaculture.
- Klíčová slova
- antibiotics, feed additives, fish diseases, immune response, probiotics,
- MeSH
- Anguilla růst a vývoj MeSH
- Bacillus subtilis MeSH
- dieta * veterinární MeSH
- Enterococcus faecium MeSH
- krmivo pro zvířata * analýza MeSH
- oligosacharidy aplikace a dávkování farmakologie MeSH
- přirozená imunita účinky léků MeSH
- synbiotika * aplikace a dávkování MeSH
- vodní hospodářství metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie veterinární MeSH
- Názvy látek
- oligosacharidy MeSH
AIM OF THE WORK: To assess the occurrence of linezolid-resistant enterococci (E. faecalis and E. faecium) in patients hospitalized at the centers and clinics of the Institute for Clinical and Experimental Medicine (IKEM). MATERIAL AND METHODS: For the period from 1. 1. 2017 to 31. 12. 2022, isolates of E. faecalis and E. faecium, which were tested for sensitivity to antibiotics, were retrospectively evaluated. Microbiological data were obtained from the laboratory information system ENVIS LIMS, and clinical data from the hospital information system IKEM. Enterococci were identified using a MALDI-TOF mass spectrometer. Susceptibility testing was performed using the disc diffusion method according to EUCAST criteria. Minimum inhibitory concentrations were determined by the Vitek automated system using the P592 card. Verification of resistance to linezolid and determination of the resistance mechanism took place at the National Reference Laboratory for Antibiotics of the National Institute of Public Health in Prague. RESULTS: In the monitored period, the sensitivity of 6900 strains of E. feacalis (67,0 %) and 3356 strains of E. faecium (33,0 %) was examined. A total of 14 linezolid-resistant enterococci (LRE) were identified - 5x E. faecalis (35,7 %) and 9x E. faecium (64,3 %). The most common mechanism of resistance to linezolid was the presence of the optrA gene in E. faecalis, and 23S rRNA mutation in E. faecium. The material with the largest LRE capture was urine (35,7 %) and secrets or punctates (28,6 %). Only in the species E. faecium, resistance to vancomycin and teicoplanin (LVRE) occurred at the same time. Three patients (21,4 %) developed an LRE infection requiring antibiotic treatment, the remaining eleven patients (78,6 %) were colonized. CONCLUSION: The proportion of linezolid-resistant enterococci was in the mentioned period low - 0,14 %. Linezolid therefore remains a safe therapeutic alternative for enterococcal infections when first-line drugs cannot be used.
- MeSH
- antibakteriální látky * farmakologie MeSH
- bakteriální léková rezistence * genetika MeSH
- Enterococcus faecalis * účinky léků genetika MeSH
- Enterococcus faecium * účinky léků genetika MeSH
- grampozitivní bakteriální infekce * mikrobiologie farmakoterapie MeSH
- lidé MeSH
- linezolid * farmakologie MeSH
- mikrobiální testy citlivosti * MeSH
- retrospektivní studie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- linezolid * MeSH
The MLST scheme currently used for Enterococcus faecium typing was designed in 2002 and is based on putative gene functions and Enterococcus faecalis gene sequences available at that time. As a result, the original MLST scheme does not correspond to the real genetic relatedness of E. faecium strains and often clusters genetically distant strains to the same sequence types (ST). Nevertheless, typing has a significant impact on the subsequent epidemiological conclusions and introduction of appropriate epidemiological measures, thus it is crucial to use a more accurate MLST scheme. Based on the genome analysis of 1,843 E. faecium isolates, a new scheme, consisting of 8 highly discriminative loci, was created in this study. These strains were divided into 421 STs using the new MLST scheme, as opposed to 223 STs assigned by the original MLST scheme. The proposed MLST has a discriminatory power of D = 0.983 (CI95% 0.981 to 0.984), compared to the original scheme's D = 0.919 (CI95% 0.911 to 0.927). Moreover, we identified new clonal complexes with our newly designed MLST scheme. The scheme proposed here is available within the PubMLST database. Although whole genome sequencing availability has increased rapidly, MLST remains an integral part of clinical epidemiology, mainly due to its high standardization and excellent robustness. In this study, we proposed and validated a new MLST scheme for E. faecium, which is based on genome-wide data and thus reflects the tested isolates' more accurate genetic similarity. IMPORTANCE Enterococcus faecium is one of the most important pathogens causing health care associated infections. One of the main reasons for its clinical importance is a rapidly spreading resistance to vancomycin and linezolid, which significantly complicates antibiotic treatment of infections caused by such resistant strains. Monitoring the spread and relationships between resistant strains causing severe conditions represents an important tool for implementing appropriate preventive measures. Therefore, there is an urgent need to establish a robust method enabling strain monitoring and comparison at the local, national, and global level. Unfortunately, the current, extensively used MLST scheme does not reflect the real genetic relatedness between individual strains and thus does not provide sufficient discriminatory power. This can lead directly to incorrect epidemiological measures due to insufficient accuracy and biased results.
- Klíčová slova
- Enterococcus faesium, clonal complex, epidemiology, multilocus sequence typing, whole genome sequenging,
- MeSH
- antibakteriální látky MeSH
- Enterococcus faecium * genetika MeSH
- grampozitivní bakteriální infekce * epidemiologie MeSH
- lidé MeSH
- multilokusová sekvenční typizace metody MeSH
- sekvenování celého genomu MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
In this study, we have focused on a multiparametric microbiological analysis of the antistaphylococcal action of the iodinated imine BH77, designed as an analogue of rafoxanide. Its antibacterial activity against five reference strains and eight clinical isolates of Gram-positive cocci of the genera Staphylococcus and Enterococcus was evaluated. The most clinically significant multidrug-resistant strains, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant S. aureus (VRSA), and vancomycin-resistant Enterococcus faecium, were also included. The bactericidal and bacteriostatic actions, the dynamics leading to a loss of bacterial viability, antibiofilm activity, BH77 activity in combination with selected conventional antibiotics, the mechanism of action, in vitro cytotoxicity, and in vivo toxicity in an alternative animal model, Galleria mellonella, were analyzed. The antistaphylococcal activity (MIC) ranged from 15.625 to 62.5 μM, and the antienterococcal activity ranged from 62.5 to 125 μM. Its bactericidal action; promising antibiofilm activity; interference with nucleic acid, protein, and peptidoglycan synthesis pathways; and nontoxicity/low toxicity in vitro and in vivo in the Galleria mellonella model were found to be activity attributes of this newly synthesized compound. In conclusion, BH77 could be rightfully minimally considered at least as the structural pattern for future adjuvants for selected antibiotic drugs. IMPORTANCE Antibiotic resistance is among the largest threats to global health, with a potentially serious socioeconomic impact. One of the strategies to deal with the predicted catastrophic future scenarios associated with the rapid emergence of resistant infectious agents lies in the discovery and research of new anti-infectives. In our study, we have introduced a rafoxanide analogue, a newly synthesized and described polyhalogenated 3,5-diiodosalicylaldehyde-based imine, that effectively acts against Gram-positive cocci of the genera Staphylococcus and Enterococcus. The inclusion of an extensive and comprehensive analysis for providing a detailed description of candidate compound-microbe interactions allows the valorization of the beneficial attributes linked to anti-infective action conclusively. In addition, this study can help with making rational decisions about the possible involvement of this molecule in advanced studies or may merit the support of studies focused on related or derived chemical structures to discover more effective new anti-infective drug candidates.
- Klíčová slova
- antibiofilm activity, antistaphylococcal activity, in vivo toxicity evaluation, iodinated imine, methicillin- and vancomycin-resistant Staphylococcus aureus,
- MeSH
- antibakteriální látky farmakologie chemie MeSH
- antiinfekční látky * farmakologie MeSH
- Enterococcus MeSH
- methicilin rezistentní Staphylococcus aureus * MeSH
- mikrobiální testy citlivosti MeSH
- rafoxanid farmakologie MeSH
- Staphylococcus aureus MeSH
- Staphylococcus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- antiinfekční látky * MeSH
- rafoxanid MeSH
