Nanoparticles have become popular in life sciences in the last few years. They have been produced in many variants and have recently been used in both biological experiments and in clinical applications. Due to concerns over nanomaterial risks, there has been a dramatic increase in investigations focused on safety research. The aim of this paper is to present the advanced testing of rhodamine-derived superparamagnetic maghemite nanoparticles (SAMN-R), which are used for their nontoxicity, biocompatibility, biodegradability, and magnetic properties. Recent results were expanded upon from the basic cytotoxic tests to evaluate cell proliferation and migration potential. Two cell types were used for the cell proliferation and tracking study: mouse embryonic fibroblast cells (3T3) and human mesenchymal stem cells (hMSCs). Advanced microscopic methods allowed for the precise quantification of the function of both cell types. This study has demonstrated that a dose of nanoparticles lower than 20 µg·cm-2 per area of the dish does not negatively affect the cells' morphology, migration, cytoskeletal function, proliferation, potential for wound healing, and single-cell migration in comparison to standard CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate). A higher dose of nanoparticles could be a potential risk for cytoskeletal folding and detachment of the cells from the solid extracellular matrix.

• Keywords
• cytotoxicity, fibroblast cells, magnetic nanoparticles, mesenchymal stem cells, single-cell migration, wound healing assay,
• MeSH
• Biomarkers MeSH
• Cell Line MeSH
• Fibroblasts drug effects   metabolism   MeSH
• Immunophenotyping MeSH
• Humans MeSH
• Magnetite Nanoparticles * chemistry   MeSH
• Mesenchymal Stem Cells drug effects   metabolism   MeSH
• Mice MeSH
• Cell Movement drug effects   MeSH
• Cell Proliferation drug effects   MeSH
• Flow Cytometry MeSH
• Reactive Oxygen Species metabolism   MeSH
• Rhodamines chemistry   pharmacology   MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Biomarkers MeSH
• Magnetite Nanoparticles * MeSH
• Reactive Oxygen Species MeSH
• Rhodamines MeSH