Membrane vesicles obtained from Acholeplasma laidlawii accumulate glucose as well as maltose and fructose against their concentration gradient in the absence of exogenous energy sources. Glucose uptake by membrane vesicles is inhibited by anaerobiosis and by electron transfer inhibitors, such as rotenone and amytal, but not by 2-heptyl-4-hydroxyquinoline N-oxide, antimycin A, cyanide and azide. Rotenone, cyanide and amytal also produce a rapid efflux of glucose from the membrane vesicles. Arsenate, oligomycin and N,N'-dicyclohexylcarbodimide do not inhibit glucose transport. Transport of glucose is markedly inhibited by proton conductors such as CCCP and pentachlorophenol. It is concluded that glucose transport can be driven by a high-energy state of the membrane or by the membrane potential.

• MeSH
• Acholeplasma laidlawii metabolism   MeSH
• Biological Transport, Active drug effects   MeSH
• Amobarbital pharmacology   MeSH
• Anaerobiosis MeSH
• Cell Membrane metabolism   MeSH
• Depression, Chemical MeSH
• Fructose metabolism   MeSH
• Glucose metabolism   MeSH
• Maltose metabolism   MeSH
• Rotenone pharmacology   MeSH
• Uncoupling Agents pharmacology   MeSH
• Subcellular Fractions MeSH
• Sulfhydryl Reagents pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Amobarbital MeSH
• Fructose MeSH
• Glucose MeSH
• Maltose MeSH
• Rotenone MeSH
• Uncoupling Agents MeSH
• Sulfhydryl Reagents MeSH