We report that the cloned DNA harboring the long terminal repeat (LTR), v-src, LTR proviral structure is tumorigenic in chickens of the Prague congenic lines. The growth rate of these tumors is by far the highest in the recombinant CC.R1 line, the B haplotype of which is composed of the B-F/L4 and B-G12 subregions originating from different naturally occurring haplotypes. Some of the tumors induced by the LTR, v-src, LTR DNA are repeatedly transplantable in syngeneic chickens, maintain unaltered provirus, and express v-src mRNA. Differences in the response to challenge with Rous sarcoma virus (RSV) and LTR, v-src, LTR DNA on a given experimental model are compared and possible involvement of an interaction between B-F/L and B-G region genes is considered. Regression of the LTR, v-src, LTR DNA-induced tumors did not prevent the formation and growth of tumors induced subsequently by RSV.

• MeSH
• Cell Division genetics   MeSH
• DNA, Neoplasm physiology   MeSH
• Genes, src physiology   MeSH
• Cloning, Molecular MeSH
• Chickens MeSH
• Neoplasms genetics   microbiology   MeSH
• Blotting, Northern MeSH
• Polymorphism, Restriction Fragment Length MeSH
• Proviruses MeSH
• Repetitive Sequences, Nucleic Acid physiology   MeSH
• Blotting, Southern MeSH
• Genetic Complementation Test MeSH
• Avian Sarcoma Viruses MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• DNA, Neoplasm MeSH

Different types of altered proviruses of Rous sarcoma virus (RSV) have been detected in mammalian tumor cell lines. We cloned and sequenced one of these altered proviruses with the structure LTR, v-src, LTR. The presence of an intact viral splice junction, as well as duplications of the chromosomal sequence GCGGGG flanking the two 2-base-pair-deleted LTRs, demonstrated reverse transcription and normal retroviral integration of src mRNA in mammalian cells. In addition, a 1-nucleotide deletion 2 bases upstream from the AAUAAA polyadenylation signal is suspected to be responsible for the absence of a poly(A) track in the src mRNA present in virions of rescued viruses.

• MeSH
• Neoplasms, Experimental microbiology   MeSH
• Cricetinae MeSH
• Tumor Cells, Cultured analysis   MeSH
• Oncogene Protein pp60(v-src) MeSH
• Oncogenes * MeSH
• Proviruses genetics   isolation & purification   MeSH
• Recombination, Genetic MeSH
• Repetitive Sequences, Nucleic Acid MeSH
• Retroviridae Proteins genetics   MeSH
• RNA-Directed DNA Polymerase metabolism   MeSH
• Genes, Viral * MeSH
• Viral Proteins metabolism   MeSH
• Avian Sarcoma Viruses genetics   isolation & purification   MeSH
• Animals MeSH
• Check Tag
• Cricetinae MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Oncogene Protein pp60(v-src) MeSH
• Retroviridae Proteins MeSH
• RNA-Directed DNA Polymerase MeSH
• Viral Proteins MeSH