Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO2 hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO2 concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both Candida species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of C. albicans and C. parapsilosis subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of Candida cells cultivated in shaken liquid cultures, under the atmospheric conditions.

• Keywords
• Candida albicans, Candida parapsilosis, Nce103p, carbonic anhydrase, cell wall, electron microscopy, localization, mass spectrometry,
• MeSH
• Cell Membrane enzymology   MeSH
• Cell Wall enzymology   MeSH
• Candida albicans enzymology   growth & development   MeSH
• Candida parapsilosis enzymology   growth & development   MeSH
• Cytosol enzymology   MeSH
• Microscopy, Electron MeSH
• Fungal Proteins metabolism   MeSH
• Mass Spectrometry MeSH
• Carbonic Anhydrases metabolism   MeSH
• Mitochondria enzymology   MeSH
• Batch Cell Culture Techniques MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Fungal Proteins MeSH
• Carbonic Anhydrases MeSH