Common Variable Immunodeficiency (CVID) is the most frequent symptomatic immune disorder characterized by reduced serum immunoglobulins. Patients often suffer from infectious and serious non-infectious complications which impact their life tremendously. The monogenic cause has been revealed in a minority of patients so far, indicating the role of multiple genes and environmental factors in CVID etiology. Using 16S and ITS rRNA amplicon sequencing, we analyzed the bacterial and fungal gut microbiota, respectively, in a group of 55 participants constituting of CVID patients and matched healthy controls including 16 case-control pairs living in the same household, to explore possible associations between gut microbiota composition and disease phenotype. We revealed less diverse and significantly altered bacterial but not fungal gut microbiota in CVID patients, which additionally appeared to be associated with a more severe disease phenotype. The factor of sharing the same household impacted both bacterial and fungal microbiome data significantly, although not as strongly as CVID diagnosis in bacterial assessment. Overall, our results suggest that gut bacterial microbiota is altered in CVID patients and may be one of the missing environmental drivers contributing to some of the symptoms and disease severity. Paired samples serving as controls will provide a better resolution between disease-related dysbiosis and other environmental confounders in future studies.

• Klíčová slova
• CVID, IgA, fungal microbiome, fungal microbiota, gut microbiome, gut microbiota, gut mycobiome, gut mycobiota,
• MeSH
• Bacteria klasifikace   genetika   imunologie   MeSH
• běžná variabilní imunodeficience imunologie   mikrobiologie   MeSH
• biodiverzita MeSH
• dospělí MeSH
• feces mikrobiologie   MeSH
• houby klasifikace   genetika   imunologie   MeSH
• imunoglobulin A krev   imunologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mykobiom * MeSH
• senioři MeSH
• střevní mikroflóra * imunologie   MeSH
• studie případů a kontrol MeSH
• zdraví rodiny MeSH
• zdravotní stav MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• imunoglobulin A MeSH