The genus Betanucleorhabdovirus includes plant viruses with negative sense, non-segmented, single-stranded RNA genomes. Here, we characterized putative novel betanucleorhabdoviruses infecting a medically important plant, elderberry. Total RNA was purified from the leaves of several plants, ribodepleted and sequenced using the Illumina platform. Sequence data analysis led to the identification of thirteen contigs of approximately 13.5 kb, showing a genome structure (3'-N-P-P3-M-G-L-5') typical of plant rhabdoviruses. The detected isolates showed 69.4 to 98.9% pairwise nucleotide identity and had the highest identity among known viruses (64.7-65.9%) with tomato betanucleorhabdovirus 2. A detailed similarity analysis and a phylogenetic analysis allowed us to discriminate the elderberry isolates into five groups, each meeting the sequence-based ICTV demarcation criterion in the Betanucleorhabdovirus genus (lower than 75% identity for the complete genome). Hence, the detected viruses appear to represent five novel, closely related betanucleorhabdoviruses, tentatively named Sambucus betanucleorhabdovirus 1 to 5.

• Klíčová slova
• genetic diversity, high-throughput sequencing, mixed infection, plant rhabdovirus,
• MeSH
• bez černý * MeSH
• fylogeneze * MeSH
• genetická variace * MeSH
• genom virový * MeSH
• listy rostlin virologie   MeSH
• nemoci rostlin * virologie   MeSH
• Rhabdoviridae genetika   izolace a purifikace   klasifikace   MeSH
• RNA virová genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• RNA virová MeSH

In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

• MeSH
• lidé MeSH
• Mononegavirales * MeSH
• viry * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH