Hyaluronan (HA) is widely used for eye drops as lubricant to counteract dry eye disease. High and low molecular weight HA are currently used in ophthalmology. However, a large portion of the current literature on friction and lubrication addresses articular (joint) cartilage. Therefore, eye drops compositions based on HA and its derivatized forms are extensively characterized providing data on the tribological and mucoadhesive properties. The physiochemical properties are investigated in buffers used commonly in eye drops formulations. The tribological investigation reveals that amphiphilic HA-C12 decreases the friction coefficient. At the same time, the combination of trehalose/HA or HAC12 enhances up to eighty-fold the mucoadhesiveness. Thus, it is predicted a prolonged residence time on the surface of the eye. The incorporation of trehalose enhances the protection of human keratinocytes (HaCaT) cells, as demonstrated in an in-vitro cell-desiccation model. The presence of trehalose increases the friction coefficient. Medium molecular weight HA shows significantly lower friction coefficient than high molecular weight HA. This research represents a first, wide array of features of diverse HA forms for eye drops contributing to increase the knowledge of these preparations. The results here presented also provide valuable information for the design of highly performing HA-formulations addressing specific needs before preclinic.

• Klíčová slova
• biotribology, eye drops, friction, hyaluronan, lubrication,
• MeSH
• adhezivita MeSH
• buněčné linie keratinocytů HaCaT MeSH
• filtrace MeSH
• hlen účinky léků   MeSH
• kyselina hyaluronová chemická syntéza   chemie   farmakologie   MeSH
• lidé MeSH
• lubrikace * MeSH
• nefelometrie a turbidimetrie MeSH
• oči účinky léků   MeSH
• oční roztoky farmakologie   MeSH
• protonová magnetická rezonanční spektroskopie MeSH
• reologie MeSH
• sterilizace MeSH
• systémy cílené aplikace léků * MeSH
• tření MeSH
• viskozita MeSH
• vysoušení MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kyselina hyaluronová MeSH
• oční roztoky MeSH