We report the draft genome sequence of Streptomyces sp. strain VITNK9, isolated from a soil sample collected in Vellore District (12.9165°N, 79.1325°E), Tamil Nadu, India, with an assembly size of 7,920,076 bp and 72.7% GC content.

• Publication type
• Journal Article MeSH

Man-made shallow fishponds in the Czech Republic have been facing high eutrophication since the 1950s. Anthropogenic eutrophication and feeding of fish have strongly affected the physicochemical properties of water and its aquatic community composition, leading to harmful algal bloom formation. In our current study, we characterized the phytoplankton community across three eutrophic ponds to assess the phytoplankton dynamics during the vegetation season. We microscopically identified and quantified 29 cyanobacterial taxa comprising non-toxigenic and toxigenic species. Further, a detailed cyanopeptides (CNPs) profiling was performed using molecular networking analysis of liquid chromatography-tandem mass spectrometry (LC-MS/MS) data coupled with a dereplication strategy. This MS networking approach, coupled with dereplication, on the online global natural product social networking (GNPS) web platform led us to putatively identify forty CNPs: fourteen anabaenopeptins, ten microcystins, five cyanopeptolins, six microginins, two cyanobactins, a dipeptide radiosumin, a cyclooctapeptide planktocyclin, and epidolastatin 12. We applied the binary logistic regression to estimate the CNPs producers by correlating the GNPS data with the species abundance. The usage of the GNPS web platform proved a valuable approach for the rapid and simultaneous detection of a large number of peptides and rapid risk assessments for harmful blooms.

• Keywords
• cyanobacteria, cyanopeptides, dereplication strategy, global natural product social networking (GNPS), harmful bloom, liquid chromatography-tandem mass spectrometry,
• MeSH
• Bacterial Toxins analysis   toxicity   MeSH
• Spectrometry, Mass, Electrospray Ionization * MeSH
• Risk Assessment MeSH
• Metabolomics MeSH
• Water Microbiology MeSH
• Microbiota MeSH
• Environmental Monitoring * MeSH
• Marine Toxins analysis   toxicity   MeSH
• Online Social Networking * MeSH
• Population Dynamics MeSH
• Seasons MeSH
• Ponds microbiology   MeSH
• Cyanobacteria classification   growth & development   metabolism   MeSH
• Harmful Algal Bloom * MeSH
• Tandem Mass Spectrometry * MeSH
• Chromatography, High Pressure Liquid * MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic MeSH
• Names of Substances
• Bacterial Toxins MeSH
• Marine Toxins MeSH