We identified and characterized 11 polymorphic microsatellite markers suitable for routine testing (three in the MHC class I sub-region, four in MHC class II and four in the MHC class III sub-region) of dromedaries and Bactrian camels. In total, 38 dromedaries and 33 Bactrian camels were genotyped, and interspecific differences were observed in the numbers of alleles and in allelic frequencies, as well as in the observed heterozygosity. These loci may be used as markers to study the adaptive genetic diversity of the MHC region in Old World camels.

• Klíčová slova
• Camelus bactrianus, Camelus dromedarius, camels, genetic diversity, major histocompatibility complex, microsatellite markers,
• Publikační typ
• časopisecké články MeSH

Dromedaries are an important livestock, used as beasts of burden and for meat and milk production. However, they can act as an intermediate source or vector for transmitting zoonotic viruses to humans, such as the Middle East respiratory syndrome coronavirus (MERS-CoV) or Crimean-Congo hemorrhagic fever virus (CCHFV). After several outbreaks of CCHFV in the Arabian Peninsula, recent studies have demonstrated that CCHFV is endemic in dromedaries and camel ticks in the United Arab Emirates (UAE). There is no apparent disease in dromedaries after the bite of infected ticks; in contrast, fever, myalgia, lymphadenopathy, and petechial hemorrhaging are common symptoms in humans, with a case fatality ratio of up to 40%. We used the in-solution hybridization capture of 100 annotated immune genes to genotype 121 dromedaries from the UAE tested for seropositivity to CCHFV. Through univariate linear regression analysis, we identified two candidate genes belonging to the innate immune system: FCAR and CLEC2B. These genes have important functions in the host defense against viral infections and in stimulating natural killer cells, respectively. This study opens doors for future research into immune defense mechanisms in an enzootic host against an important zoonotic disease.

• Klíčová slova
• Camelus dromedarius, Old World camel, in-solution hybridization capture, tick, vector-borne infection, zoonosis,
• MeSH
• genetická predispozice k nemoci genetika   MeSH
• genotyp MeSH
• hemoragická horečka krymská genetika   imunologie   virologie   MeSH
• infestace klíšťaty imunologie   parazitologie   MeSH
• klíšťata imunologie   fyziologie   virologie   MeSH
• koronavirové infekce genetika   imunologie   virologie   MeSH
• kuřecí embryo MeSH
• lidé MeSH
• odolnost vůči nemocem genetika   imunologie   MeSH
• přirozená imunita genetika   imunologie   MeSH
• rizikové faktory MeSH
• velbloudi genetika   imunologie   virologie   MeSH
• virus krymsko-konžské hemoragické horečky imunologie   fyziologie   MeSH
• zoonózy genetika   imunologie   virologie   MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Spojené arabské emiráty MeSH