The resistance of the emerging human pathogen Stenotrophomonas maltophilia to tetracycline antibiotics mainly depends on multidrug efflux pumps and ribosomal protection enzymes. However, the genomes of several strains of this Gram-negative bacterium code for a FAD-dependent monooxygenase (SmTetX) homologous to tetracycline destructases. This protein was recombinantly produced and its structure and function were investigated. Activity assays using SmTetX showed its ability to modify oxytetracycline with a catalytic rate comparable to those of other destructases. SmTetX shares its fold with the tetracycline destructase TetX from Bacteroides thetaiotaomicron; however, its active site possesses an aromatic region that is unique in this enzyme family. A docking study confirmed tetracycline and its analogues to be the preferred binders amongst various classes of antibiotics.

• Klíčová slova
• FAD-dependent monooxygenases, antibiotic resistance, tetracycline,
• MeSH
• antibakteriální látky farmakologie   chemie   MeSH
• krystalografie rentgenová MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• oxytetracyklin * metabolismus   MeSH
• Stenotrophomonas maltophilia * genetika   metabolismus   MeSH
• tetracyklin farmakologie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• oxytetracyklin * MeSH
• tetracyklin MeSH

The synchrotron facilities used in collecting the data for the article by Švecová et al. [(2021), Acta Cryst. D77, 755-775] are acknowledged.

• Klíčová slova
• Chaetomium thermophilum, FAD-dependent oxidoreductases, GMC oxidoreductases, corrigendum, crystallographic fragment screening, synchrotron facilities,
• Publikační typ
• tisková chyba MeSH

In macromolecular crystallography, paired refinement is generally accepted to be the optimal approach for the determination of the high-resolution cutoff. The software tool PAIREF provides automation of the protocol and associated analysis. Support for phenix.refine as a refinement engine has recently been implemented in the program. This feature is presented here using previously published data for thermolysin. The results demonstrate the importance of the complete cross-validation procedure to obtain a thorough and unbiased insight into the quality of high-resolution data.

• Klíčová slova
• PAIREF, Phenix, X-ray diffraction, high-resolution limit, macromolecular crystallography, paired refinement,
• MeSH
• databáze proteinů * normy   MeSH
• krystalografie rentgenová metody   normy   MeSH
• software * normy   MeSH
• Publikační typ
• časopisecké články MeSH