Binder H33 is a small protein binder engineered by ribosome display to bind human interleukin 10. Crystals of binder H33 display severe diffraction anisotropy. A set of data files with correction for diffraction anisotropy based on different local signal-to-noise ratios was prepared. Paired refinement was used to find the optimal anisotropic high-resolution diffraction limit of the data: 3.13-2.47 Å. The structure of binder H33 belongs to the 2% of crystal structures with the highest solvent content in the Protein Data Bank.

• Keywords
• anisotropy, binder H33, paired refinement,
• Publication type
• Journal Article MeSH

The resistance of the emerging human pathogen Stenotrophomonas maltophilia to tetracycline antibiotics mainly depends on multidrug efflux pumps and ribosomal protection enzymes. However, the genomes of several strains of this Gram-negative bacterium code for a FAD-dependent monooxygenase (SmTetX) homologous to tetracycline destructases. This protein was recombinantly produced and its structure and function were investigated. Activity assays using SmTetX showed its ability to modify oxytetracycline with a catalytic rate comparable to those of other destructases. SmTetX shares its fold with the tetracycline destructase TetX from Bacteroides thetaiotaomicron; however, its active site possesses an aromatic region that is unique in this enzyme family. A docking study confirmed tetracycline and its analogues to be the preferred binders amongst various classes of antibiotics.

• Keywords
• FAD-dependent monooxygenases, antibiotic resistance, tetracycline,
• MeSH
• Anti-Bacterial Agents pharmacology   chemistry   MeSH
• Crystallography, X-Ray MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Oxytetracycline * metabolism   MeSH
• Stenotrophomonas maltophilia * genetics   metabolism   MeSH
• Tetracycline pharmacology   metabolism   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Oxytetracycline * MeSH
• Tetracycline MeSH