Small secreted proteins play an important role in plant development, as well as in reactions to changes in the environment. In Arabidopsis thaliana, they are predominantly members of highly expanded families, such as the pathogenesis-related (PR) 1-like protein family, whose most studied member PR1 is involved in plant defense responses by a so far unknown mechanism, or Clavata3/Endosperm Surrounding Region (CLE) protein family, whose members' functions in the development are well described. Our survey of the existing literature for the two families showed a lack of details on their localization, trafficking, and exocytosis. Therefore, in order to uncover the modes of their secretion, we tested the hypothesis that a direct link between the secreted cargoes and the secretion regulators such as Rab GTPases, SNAREs, and exocyst subunits could be established using in silico co-expression and clustering approaches. We employed several independent techniques to uncover that only weak co-expression links could be found for limited numbers of secreted cargoes and regulators. We propose that there might be particular spatio-temporal requirements for PR1 and CLE proteins to be synthesized and secreted, and efforts to experimentally cover these discrepancies should be invested along with functional studies.

• Klíčová slova
• CLE, PR1, SNARE, co-expression, exocyst, secretion,
• MeSH
• Arabidopsis * genetika   metabolismus   MeSH
• cytoplazma metabolismus   MeSH
• exocytóza fyziologie   MeSH
• proteiny huseníčku * genetika   metabolismus   MeSH
• proteiny SNARE metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• proteiny huseníčku * MeSH
• proteiny SNARE MeSH

INTRODUCTION: The yield of chickpea is severely hampered by infection wilt caused by several races of Fusarium oxysporum f. sp. ciceris (Foc). METHODS: To understand the underlying molecular mechanisms of resistance against Foc4 Fusarium wilt, RNA sequencing-based shoot transcriptome data of two contrasting chickpea genotypes, namely KWR 108 (resistant) and GL 13001 (susceptible), were generated and analyzed. RESULTS AND DISCUSSION: The shoot transcriptome data showed 1,103 and 1,221 significant DEGs in chickpea genotypes KWR 108 and GL 13001, respectively. Among these, 495 and 608 genes were significantly down and up-regulated in genotypes KWR 108, and 427 and 794 genes were significantly down and up-regulated in genotype GL 13001. The gene ontology (GO) analysis of significant DEGs was performed and the GO of the top 50 DEGs in two contrasting chickpea genotypes showed the highest cellular components as membrane and nucleus, and molecular functions including nucleotide binding, metal ion binding, transferase, kinase, and oxidoreductase activity involved in biological processes such as phosphorylation, oxidation-reduction, cell redox homeostasis process, and DNA repair. Compared to the susceptible genotype which showed significant up-regulation of genes involved in processes like DNA repair, the significantly up-regulated DEGs of the resistant genotypes were involved in processes like energy metabolism and environmental adaptation, particularly host-pathogen interaction. This indicates an efficient utilization of environmental adaptation pathways, energy homeostasis, and stable DNA molecules as the strategy to cope with Fusarium wilt infection in chickpea. The findings of the study will be useful in targeting the genes in designing gene-based markers for association mapping with the traits of interest in chickpea under Fusarium wilt which could be efficiently utilized in marker-assisted breeding of chickpea, particularly against Foc4 Fusarium wilt.

• Klíčová slova
• Fusarium wilt, RNA sequencing, chickpea, energy metabolism, environmental adaptation, transcriptome,
• Publikační typ
• časopisecké články MeSH