Besides many other uses, dried Cannabis may be used for "tea" preparation. This study focused on a comprehensive characterization of an aqueous infusion prepared according to a common practice from three fairly different Cannabis cultivars. The transfer of 42 phytocannabinoids and 12 major bioactive compounds (flavonoids) into the infusion was investigated using UHPLC-HRMS/MS. Phytocannabinoid acids were transferred generally in a higher extent compared to their counterparts; in the case of Δ9-THC, it was only in the range of 0.4-1.9% of content in the Cannabis used. A dramatic increase of phytocannabinoids, mainly of the neutral species, occurred when cream was added during steeping, and the transfer of Δ9-THC into "tea" achieved a range of 53-64%. Under such conditions, drinking a 250 mL cup of such tea by a 70 kg person might lead to multiple exceedance of the Acute Reference Dose (ARfD), 1 μg/kg b.w., even in the case when using hemp with a Δ9-THC content below 1% in dry weight for preparation.

• Keywords
• THC isomers, UHPLC-HRMS/MS, bioactive compounds, cannabis tea, cannflavins, flavonoids, phytocannabinoids,
• MeSH
• Tea chemistry   MeSH
• Cannabis * chemistry   MeSH
• Flavonoids chemistry   analysis   MeSH
• Cannabinoids analysis   chemistry   MeSH
• Humans MeSH
• Plant Extracts chemistry   MeSH
• Tandem Mass Spectrometry MeSH
• Dronabinol analysis   chemistry   MeSH
• Chromatography, High Pressure Liquid MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Tea MeSH
• Flavonoids MeSH
• Cannabinoids MeSH
• Plant Extracts MeSH
• Dronabinol MeSH