Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic molecules, such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these molecules can affect physiological processes, the main aim of this study was to explore the potential biological impacts of fluorescein photochemistry. In our in vitro study in a human hepatoblastoma HepG2 cell line, we explored the possible effects on cell viability, cellular energy metabolism, and the cell cycle. We observed markedly lowered cell viability (≈30%, 75-2400 μM) upon irradiation of intracellular fluorescein and proved that this decrease in viability was dependent on the cellular oxygen concentration. We also detected a significantly decreased concentration of Krebs cycle metabolites (lactate and citrate < 30%; 2-hydroxyglutarate and 2-oxoglutarate < 10%) as well as cell cycle arrest (decrease in the G2 phase of 18%). These observations suggest that this photochemical reaction could have important biological consequences and may account for some adverse reactions observed in fluorescein-treated patients. Additionally, the biological activities of both 1O2 and CO might have considerable therapeutic potential, particularly in the treatment of cancer.

• Klíčová slova
• carbon monoxide, fluorescein, irradiation, metabolism, proliferation, singlet oxygen, viability,
• MeSH
• angiografie MeSH
• buňky Hep G2 MeSH
• citrátový cyklus účinky léků   účinky záření   MeSH
• fluorescein chemie   farmakologie   MeSH
• fotochemické procesy MeSH
• kontrolní body buněčného cyklu účinky léků   účinky záření   MeSH
• lidé MeSH
• oxid uhelnatý analýza   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
• protinádorové látky chemie   farmakologie   MeSH
• singletový kyslík analýza   MeSH
• světlo MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fluorescein MeSH
• oxid uhelnatý MeSH
• protinádorové látky MeSH
• singletový kyslík MeSH