Most cited article - PubMed ID 37690699
Synthetic and analytical routes to the L-amino acid conjugates of cis-OPDA and their identification and quantification in plants
Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating "defense versus growth" responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood. An isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected years ago in wounded leaves of flowering plants, opening up the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp accumulating in response to biotic and abiotic stress in Arabidopsis (Arabidopsis thaliana). The OPDA amino acid conjugates displayed cis-OPDA-related plant responses in a JA-Ile-dependent manner. We also showed that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are mediated by members of the amidosynthetase GRETCHEN HAGEN 3 and the amidohydrolase INDOLE-3-ACETYL-LEUCINE RESISTANT 1/ILR1-like families. Thus, OPDA amino acid conjugates function in the catabolism or temporary storage of cis-OPDA in stress responses instead of acting as chemical signals per se.
- MeSH
- Amides metabolism MeSH
- Arabidopsis * genetics metabolism MeSH
- Cyclopentanes * metabolism MeSH
- Stress, Physiological * MeSH
- Homeostasis * MeSH
- Isoleucine analogs & derivatives metabolism MeSH
- Fatty Acids, Unsaturated * metabolism MeSH
- Oxylipins * metabolism MeSH
- Gene Expression Regulation, Plant MeSH
- Plant Growth Regulators metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 12-oxophytodienoic acid MeSH Browser
- Amides MeSH
- Cyclopentanes * MeSH
- Isoleucine MeSH
- jasmonic acid MeSH Browser
- Fatty Acids, Unsaturated * MeSH
- Oxylipins * MeSH
- Plant Growth Regulators MeSH
