In response to environmental changes, plants continuously make architectural changes in order to optimize their growth and development. The regulation of plant branching, influenced by environmental conditions and affecting hormone balance and gene expression, is crucial for agronomic purposes due to its direct correlation with yield. Strigolactones (SL), the youngest class of phytohormones, function to shape the architecture of plants by inhibiting axillary outgrowth. Barley plants harboring the mutation in the HvDWARF14 (HvD14) gene, which encodes the SL-specific receptor, produce almost twice as many tillers as wild-type (WT) Sebastian plants. Here, through hormone profiling and comparison of transcriptomic and proteomic changes between 2- and 4-week-old plants of WT and hvd14 genotypes, we elucidate a regulatory mechanism that might affect the tillering of SL-insensitive plants. The analysis showed statistically significant increased cytokinin content and decreased auxin and abscisic acid content in 'bushy' hvd14 compared to WT, which aligns with the commonly known actions of these hormones regarding branching regulation. Further, transcriptomic and proteomic analysis revealed a set of differentially expressed genes (DEG) and abundant proteins (DAP), among which 11.6% and 14.6% were associated with phytohormone-related processes, respectively. Bioinformatics analyses then identified a series of potential SL-dependent transcription factors (TF), which may control the differences observed in the hvd14 transcriptome and proteome. Comparison to available Arabidopsis thaliana data implicates a sub-selection of these TF as being involved in the transduction of SL signal in both monocotyledonous and dicotyledonous plants.

• Klíčová slova
• Hordeum vulgare, Branching, Phytohormone cross-talk, Strigolactones,
• MeSH
• cytokininy metabolismus   MeSH
• heterocyklické sloučeniny tricyklické * MeSH
• homeostáza MeSH
• ječmen (rod) * růst a vývoj   metabolismus   MeSH
• laktony * metabolismus   MeSH
• mutace MeSH
• proteom analýza   MeSH
• regulace genové exprese u rostlin MeSH
• regulátory růstu rostlin * metabolismus   MeSH
• rostlinné proteiny genetika   metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• transkripční faktory metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cytokininy MeSH
• GR24 strigolactone MeSH Prohlížeč
• heterocyklické sloučeniny tricyklické * MeSH
• laktony * MeSH
• proteom MeSH
• regulátory růstu rostlin * MeSH
• rostlinné proteiny MeSH
• transkripční faktory MeSH