This study explores the innovative approach in the development of freeze-dried hydrogel films, leveraging the unique properties of gum Karaya (GK), poly-(vinyl alcohol) (PVA), poly-(ethylene glycol) (PEG), and glycerol with a coating of octenidine dihydrochloride (OCT). These innovative hydrogel films exhibit at a certain glycerol concentration a sandwich-like structure, achieved through a tailored freeze-drying process, which enhances transparency and mechanical stability. OCT provides superior antibacterial performance, effectively combating multidrug-resistant bacteria with a controlled and gradual release mechanism, surpassing conventional OCT solutions that require frequent reapplication for infected wound treatment without the creation of bacterial resistance. Advanced environmental scanning electron microscopy (A-ESEM) reveals the complex microstructure of the hydrogel, highlighting the dense surface layer and interconnected porous bulk. Variations in glycerol concentrations proved to significantly impact hydrogels' properties. Increasing the glycerol concentration decreases the pore size (around 4.5 μm) while enhancing the polymer network density and flexibility. However, low concentration increases the pore size (7.8-15.6 μm), impacting enhanced swelling behavior and hydrolytic stability. OCT's rapid antibacterial action, releasing over 30% within the first hour and maintaining prolonged activity for up to 2 weeks, emphasizes the material's potential for diverse applications. Hydrogels' remarkable transparency, porosity, structural stability, and antibacterial efficacy against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli strains suggest promising uses as transparent dressings, biomedical devices, and infection-resistant surfaces.

• Publikační typ
• časopisecké články MeSH

Multifunctional polymers are interesting substances for the formulation of drug molecules that cannot be administered in their pure form due to their pharmacokinetic profiles or side effects. Polymer-drug formulations can enhance pharmacological properties or create tissue specificity by encapsulating the drug into nanocontainers, or stabilizing nanoparticles for drug transport. We present the synthesis of multifunctional poly(2-ethyl-2-oxazoline-co-2-glyco-2-oxazoline)s containing two reactive end groups, and an additional hydrophobic anchor at one end of the molecule. These polymers were successfully used to stabilize (solid) lipid nanoparticles ((S)LNP) consisting of tetradecan-1-ol and cholesterol with their hydrophobic anchor. While the pure polymers interacted with GLUT1-expressing cell lines mainly based on their physicochemical properties, especially via interactions of the hydrophobic anchor with membranous compartments of the cells, LNP-cell interactions hinted toward an influence of the glucosylation on particle-cell interactions. The presented LNP are therefore promising systems for the delivery of drugs into GLUT1-expressing cell lines.

• MeSH
• hydrofobní a hydrofilní interakce MeSH
• lidé MeSH
• lipidy * chemie   MeSH
• nanočástice * chemie   MeSH
• nosiče léků chemie   MeSH
• oxazoly * chemie   MeSH
• polymery * chemie   MeSH
• přenašeč glukosy typ 1 metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• lipidy * MeSH
• nosiče léků MeSH
• oxazoly * MeSH
• poly(2-oxazoline) MeSH Prohlížeč
• polymery * MeSH
• přenašeč glukosy typ 1 MeSH
• SLC2A1 protein, human MeSH Prohlížeč