Influence of intestinal colonization by a probiotic E. coli strain on the incidence of bacterial pathogens in stool and allergic symptoms during the 1st year of life was monitored in 3 groups: colonized children of allergic mothers (AC; n = 52), noncolonized children of allergic mothers (AN; n = 50), children of nonallergic mothers (NC; n = 42). Colinfant vaccine was administered within 2 d after birth, 3 x a week over a period of 4 weeks. Stool samples were examined after 2 d and at the age of 3, 6 and 12 months. At 3 months E. coli was present in 90 %, at 12 months in 73 % of AC. Pathogens were significantly less frequent on day 3 and at 3 months in AC vs. AN (15 vs. 61 %, p < 0.001; 15 vs. 38 %, p < 0.01) and vs. NC (15 vs. 63 %, p < 0.001; 15 vs. 53 %, p < 0.001). AC exhibited lower incidence of Staphylococcus epidermidis than AN on day 3 (6 vs. 31 %, p < 0.001) and of Klebsiella strains on day 3 and at 3 months (4 vs. 20 %, p < 0.05; 5 vs. 24 %, p < 0.01). AC showed a lower incidence of Pseudomonas aeruginosa than NC on day 3 (6 vs. 31 %, p < 0.01) and Klebsiella spp. on day 3 and at 3 months (4 vs. 22 %, p < 0.05; 5 vs. 45 %, p < 0.001). No significant differences were recorded after 6 and 12 months. The incidence of allergies was 3 % in AC, 26 % in AN (p < 0.01), and 10 % in NC.

• MeSH
• alergie etiologie   prevence a kontrola   MeSH
• aplikace orální MeSH
• bakteriální infekce komplikace   mikrobiologie   prevence a kontrola   MeSH
• Escherichia coli * MeSH
• incidence MeSH
• Klebsiella izolace a purifikace   MeSH
• kojenec MeSH
• lidé MeSH
• následné studie MeSH
• nemoci střev komplikace   mikrobiologie   prevence a kontrola   MeSH
• novorozenec MeSH
• přenašečství MeSH
• probiotika aplikace a dávkování   MeSH
• Pseudomonas aeruginosa izolace a purifikace   MeSH
• Staphylococcus epidermidis izolace a purifikace   MeSH
• střeva mikrobiologie   MeSH
• zdravotnické přehledy * MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Thirty-three fully breast-fed infants aged between 1 and 12 weeks were screened for bifidobacteria in feces. Bifidobacteria counts in most fecal samples determined both by TPY agar and FISH procedure ranged from 10(8) to 10(11) CFU/g. Three infants did not contain any bifidobacteria in their fecal samples. One child was delivered by caesarean section and the other two by normal vaginal delivery. All bifidobacteria-free infants possessed Gram-positive regular rods as a major group of their fecal flora. These bacteria were identified as clostridia using genus-specific FISH probe. In bifidobacteria-positive samples, B. longum (57.9% of the samples) was the most frequently found species, followed by B. adolescentis (31.6%), B. bifidum (21.0%), B. breve (10.5%), B. pseudocatenulatum (5.3%), and B. dentium (5.3%).

• MeSH
• Bifidobacterium genetika   růst a vývoj   izolace a purifikace   MeSH
• Clostridium izolace a purifikace   MeSH
• feces mikrobiologie   MeSH
• hybridizace in situ fluorescenční MeSH
• kojenec MeSH
• kojení MeSH
• lidé MeSH
• novorozenec MeSH
• počet mikrobiálních kolonií MeSH
• Check Tag
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH