Samples of chernozem soil were enriched with vanillic acid, protocatechuic acid glucose, a mixture of glucose and (NH4)2SO4 (C : N = 5 : 1), ethanol and 2,4-dichlorophenoxyacetic acid (2,4-D). After a 6-d (with 2,4-D 35-d) incubation during which primary oxidation of the introduced substrates occurred, the soil was supplied with a solution of 2-14C-2,4-D (50 ppm; 6.7 kBq) and production of 14CO2 (product of microbial degradation of 2,4-D) was measured. Previously enriched samples exhibited a higher degradation rate; both the lag phase and doubling time of mineralization activity in the exponential phase of the process were markedly higher. This reflected an overall proliferation of bacteria and the increased relative proportion of bacterial strains capable of mineralizing 2,4-D in enriched samples. The stimulation of 2,4-D degradation may involve specific adaptation and selection mechanisms (as in the case with samples previously enriched with 2,4-D or its structural analogues--aromatic monomers, ethanol) as well as non-specific mechanisms. The extent of mineralization of 2,4-D was not affected by soil pretreatment, about 1/3 of introduced radioactive carbon being invariably transformed to 14CO2.

• MeSH
• ethanol farmakologie   MeSH
• glukosa farmakologie   MeSH
• hydroxybenzoáty farmakologie   MeSH
• kyselina 2,4-dichlorfenoxyoctová metabolismus   MeSH
• kyselina vanilová farmakologie   MeSH
• oxid uhličitý metabolismus   MeSH
• půdní mikrobiologie * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• ethanol MeSH
• glukosa MeSH
• hydroxybenzoáty MeSH
• kyselina 2,4-dichlorfenoxyoctová MeSH
• kyselina vanilová MeSH
• oxid uhličitý MeSH
• protocatechuic acid MeSH Prohlížeč

Mineralization of native organic matter and U-14C-glucose was followed by measuring the formation of CO2 and its radioactivity in chernozem soil presterilized by gamma-radiation and inoculated with a washed suspension of Cellulomonas sp. cells. The introduced bacteria mineralized the soil organic component to a higher extent in variants enriched with glucose. This so-called priming effect of glucose was observed also in the presence of chloramphenicol, inhibiting the growth of the bacteria. The increased mineralization of the native soil organic fraction was also detected in samples that were not enriched with glucose when the bacterial suspension was first disintegrated ultrasonically and the material then used for the inoculation. Possible participation of phenomena of the type of cometabolism and activation of cell membrane transport mechanisms on the occurrence of the priming effect of glucose in the soil is discussed.

• MeSH
• Actinomycetales účinky léků   růst a vývoj   metabolismus   MeSH
• chemická deprese MeSH
• chloramfenikol farmakologie   MeSH
• glukosa farmakologie   MeSH
• kultivační techniky MeSH
• oxid uhličitý biosyntéza   MeSH
• průmyslová hnojiva MeSH
• půdní mikrobiologie * MeSH
• sterilizace MeSH
• záření gama MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chloramfenikol MeSH
• glukosa MeSH
• oxid uhličitý MeSH
• průmyslová hnojiva MeSH

• MeSH
• Bacillus cereus metabolismus   MeSH
• Bacteria metabolismus   MeSH
• benzoáty metabolismus   MeSH
• druhová specificita MeSH
• glukosa metabolismus   MeSH
• manometrie MeSH
• oxidace-redukce MeSH
• Pseudomonas metabolismus   MeSH
• půdní mikrobiologie * MeSH
• radiační účinky * MeSH
• spotřeba kyslíku účinky záření   MeSH
• sterilizace MeSH
• vysoká teplota * MeSH
• Xanthomonas metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• benzoáty MeSH
• glukosa MeSH