Caldimonas thermodepolymerans, a Gram-negative, moderately thermophilic bacterium, exhibits a remarkable biotechnological potential. Given the presence of genes in its genome dedicated to the metabolization of ferulic acid (FA), this study aimed to explore the bacterium's capability for biotransforming FA into high-value metabolites. The results unequivocally demonstrate the bacterium's proficiency in the efficient and rapid conversion of FA into vanillyl alcohol (VOH) and vanillic acid (VA). By manipulating key cultivation parameters, such as adjusting initial FA doses and varying cultivation periods, the product profile can be tailored. Higher initial doses and shorter cultivation periods favor the production of VOH, while lower FA doses and extended cultivation periods lead to the predominant formation of VA. Furthermore, the process can be operated in a repeated-batch scenario. This underscores the potential of C. thermodepolymerans for industrial biotransformation of FA, presenting a promising avenue for leveraging its capabilities in practical applications.
- Klíčová slova
- Biotransformation, Caldimonas thermodepolymerans, Ferulic acid, Thermophiles, Vanillic acid, Vanillyl alcohol,
- MeSH
- benzylalkoholy * metabolismus MeSH
- biotransformace * MeSH
- kyselina vanilová * metabolismus MeSH
- kyseliny kumarové * metabolismus MeSH
- průmyslová mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzylalkoholy * MeSH
- ferulic acid MeSH Prohlížeč
- kyselina vanilová * MeSH
- kyseliny kumarové * MeSH
- vanillyl alcohol MeSH Prohlížeč
Human phospholipase A₂ (hPLA₂) of the IIA group (HGIIA) catalyzes the hydrolysis of membrane phospholipids, producing arachidonic acid and originating potent inflammatory mediators. Therefore, molecules that can inhibit this enzyme are a source of potential anti-inflammatory drugs, with different action mechanisms of known anti-inflammatory agents. For the study and development of new anti-inflammatory drugs with this action mechanism, snake venom PLA₂ (svPLA₂) can be employed, since the svPLA₂ has high similarity with the human PLA₂ HGIIA. Despite the high similarity between these secretory PLA₂s, it is still not clear if these toxins can really be employed as an experimental model to predict the interactions that occur with the human PLA₂ HGIIA and its inhibitors. Thus, the present study aims to compare and evaluate, by means of theoretical calculations, docking and molecular dynamics simulations, as well as experimental studies, the interactions of human PLA₂ HGIIA and two svPLA₂s,Bothrops toxin II and Crotoxin B (BthTX-II and CB, respectively). Our theoretical findings corroborate experimental data and point out that the human PLA₂ HGIIA and svPLA₂ BthTX-II lead to similar interactions with the studied compounds. From our results, the svPLA₂ BthTX-II can be used as an experimental model for the development of anti-inflammatory drugs for therapy in humans.
- Klíčová slova
- experimental model, svPLA2, vanillic acid,
- MeSH
- antiflogistika chemie MeSH
- crotoxin metabolismus MeSH
- fosfolipasy A2, skupina II MeSH
- fosfolipasy A2 chemie MeSH
- inhibitory fosfolipasy A2 chemie MeSH
- jedy chřestýšů enzymologie MeSH
- kyselina vanilová chemie MeSH
- lidé MeSH
- sekvence aminokyselin MeSH
- simulace molekulového dockingu MeSH
- vodíková vazba MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antiflogistika MeSH
- bothropstoxin II MeSH Prohlížeč
- crotoxin MeSH
- fosfolipasy A2, skupina II MeSH
- fosfolipasy A2 MeSH
- inhibitory fosfolipasy A2 MeSH
- jedy chřestýšů MeSH
- kyselina vanilová MeSH
This study investigated the effect of one-week consumption of 165 g/day fresh blue honeysuckle berries (208 mg/day anthocyanins) in 10 healthy volunteers. At the end of intervention, levels of benzoic (median 1782 vs 4156), protocatechuic (709 vs 2417), vanillic (2779 vs 4753), 3-hydroxycinnamic (143 vs 351), p-coumaric (182 vs 271), isoferulic (805 vs 1570), ferulic (1086 vs 2395), and hippuric (194833 vs 398711 μg/mg creatinine) acids by LC/MS were significantly increased in the urine. Clinical chemistry safety markers were not altered. Oxidative stress markers, erythrocyte glutathione peroxidase (0.73 vs 0.88 U/g Hb) and catalase (2.5 vs 2.8 μkat/g Hb) activities, and erythrocyte/plasma thiobarbituric acid reactive substance (522 vs 612/33 vs 38 μmol/g Hb/protein) levels were significantly increased, without change in plasma antioxidant status. Nonsignificant changes of advanced oxidation protein products and oxidized LDL were observed. The results provide a solid base for further study of metabolite excretion and antioxidant parameters after ingestion of anthocyanins.
- MeSH
- anthokyaniny aplikace a dávkování MeSH
- antioxidancia metabolismus MeSH
- biologické markery moč MeSH
- chromatografie kapalinová MeSH
- cinnamáty moč MeSH
- dospělí MeSH
- erytrocyty metabolismus MeSH
- glutathionperoxidasa krev MeSH
- hippuráty moč MeSH
- hmotnostní spektrometrie MeSH
- hydroxybenzoáty moč MeSH
- katalasa krev MeSH
- kyselina benzoová moč MeSH
- kyselina vanilová moč MeSH
- kyseliny kumarové moč MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- lidé MeSH
- lipoproteiny LDL krev MeSH
- Lonicera chemie MeSH
- metabolom * MeSH
- ovoce chemie MeSH
- oxidační stres účinky léků MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- anthokyaniny MeSH
- antioxidancia MeSH
- biologické markery MeSH
- cinnamáty MeSH
- glutathionperoxidasa MeSH
- hippuráty MeSH
- hippuric acid MeSH Prohlížeč
- hydroxybenzoáty MeSH
- isoferulic acid MeSH Prohlížeč
- katalasa MeSH
- kyselina benzoová MeSH
- kyselina vanilová MeSH
- kyseliny kumarové MeSH
- látky reagující s kyselinou thiobarbiturovou MeSH
- lipoproteiny LDL MeSH
- oxidized low density lipoprotein MeSH Prohlížeč
- phenolic acid MeSH Prohlížeč
- protocatechuic acid MeSH Prohlížeč
Complete separation of aglycones and glucosides of selected isoflavones (genistin, genistein, daidzin, daidzein, glycitin, glycitein, ononin, sissotrin, formononetin, and biochanin A) was possible in 1.5 min using an ultrahigh-pressure liquid chromatography (U-HPLC) on a different particular chemically modified stationary phases with a particle size under 2 microm. In addition, selected separation conditions for simultaneous determination of isoflavones together with a group of phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, and sinapic acid) allowed separation of all 19 compounds in 1.9 min. Separations were conducted on a non-polar reversed phase (C(18)) and also on more polar phases with cyanopropyl or phenyl groups using a gradient elution with a mobile phase consisting of 0.3% aqueous acetic acid and methanol. Chromatographic peaks were characterised using parameters such as resolution, symmetry, selectivity, etc. Individual substances were identified and quantified using UV-vis diode array detector at wavelength 270 nm. Limits of detection (3S/N) were in the range 200-400 pg ml(-1). Proposed U-HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials (Trifolium pratense, Glycine max, Pisum sativum and Ononis spinosa) after acid hydrolysis of the samples and modified Soxhlet extraction.
- MeSH
- genistein chemie izolace a purifikace MeSH
- Glycine max chemie MeSH
- hrách setý chemie MeSH
- hydroxybenzoáty chemie izolace a purifikace MeSH
- isoflavony chemie izolace a purifikace MeSH
- kyselina gallová analogy a deriváty chemie izolace a purifikace MeSH
- kyselina vanilová chemie izolace a purifikace MeSH
- kyseliny kávové chemie izolace a purifikace MeSH
- kyseliny kumarové chemie izolace a purifikace MeSH
- molekulární struktura MeSH
- propionáty MeSH
- rostlinné extrakty chemie izolace a purifikace MeSH
- Trifolium chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biochanin A MeSH Prohlížeč
- caffeic acid MeSH Prohlížeč
- daidzein MeSH Prohlížeč
- daidzin MeSH Prohlížeč
- ferulic acid MeSH Prohlížeč
- formononetin MeSH Prohlížeč
- genistein MeSH
- genistin MeSH Prohlížeč
- glycitein MeSH Prohlížeč
- glycitin MeSH Prohlížeč
- hydroxybenzoáty MeSH
- isoflavony MeSH
- kyselina gallová MeSH
- kyselina vanilová MeSH
- kyseliny kávové MeSH
- kyseliny kumarové MeSH
- p-coumaric acid MeSH Prohlížeč
- phenolic acid MeSH Prohlížeč
- propionáty MeSH
- protocatechuic acid MeSH Prohlížeč
- rostlinné extrakty MeSH
- sinapinic acid MeSH Prohlížeč
- syringic acid MeSH Prohlížeč
Macroporous poly(styrene-divinylbenzene) monolithic columns were prepared in fused silica capillaries of 100 microm id by in-situ copolymerization of styrene with divinylbenzene in the presence of propan-1-ol and formamide as the porogen system. The monoliths were subsequently alkylated with linear alkyl C-18 groups via Friedel-Crafts reaction to improve the retention and chromatographic resolution of strongly polar phenolic acids. A new thermally initiated grafting procedure was developed in order to shorten the time of the alkylation process. The grafting procedure was optimized with respect to the reaction temperature, time, the grafting reactant concentration, and the solvent used. The type of solvent and the grafting temperature are the most significant factors affecting the hydrodynamic properties, porosity, and efficiency of the columns. While the equivalent particle diameter of the grafted column increased, the capillary-like flow-through pore diameter decreased in comparison to non-alkylated monoliths. The hydrodynamic permeability of the monolith decreased, but the monolithic column still permitted fast micro-HPLC separations.
- MeSH
- alkylace MeSH
- časové faktory MeSH
- hydroxybenzoáty analýza MeSH
- kapilární elektrochromatografie přístrojové vybavení metody MeSH
- kyselina gallová analogy a deriváty analýza MeSH
- kyselina vanilová analýza MeSH
- kyseliny kumarové analýza MeSH
- polystyreny chemická syntéza chemie MeSH
- poréznost MeSH
- povrchové vlastnosti MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- velikost částic MeSH
- vysokoúčinná kapalinová chromatografie přístrojové vybavení metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Amberlite XAD-2 resin MeSH Prohlížeč
- ferulic acid MeSH Prohlížeč
- hydroxybenzoáty MeSH
- kyselina gallová MeSH
- kyselina vanilová MeSH
- kyseliny kumarové MeSH
- phenolic acid MeSH Prohlížeč
- polystyreny MeSH
- syringic acid MeSH Prohlížeč
Samples of chernozem soil were enriched with vanillic acid, protocatechuic acid glucose, a mixture of glucose and (NH4)2SO4 (C : N = 5 : 1), ethanol and 2,4-dichlorophenoxyacetic acid (2,4-D). After a 6-d (with 2,4-D 35-d) incubation during which primary oxidation of the introduced substrates occurred, the soil was supplied with a solution of 2-14C-2,4-D (50 ppm; 6.7 kBq) and production of 14CO2 (product of microbial degradation of 2,4-D) was measured. Previously enriched samples exhibited a higher degradation rate; both the lag phase and doubling time of mineralization activity in the exponential phase of the process were markedly higher. This reflected an overall proliferation of bacteria and the increased relative proportion of bacterial strains capable of mineralizing 2,4-D in enriched samples. The stimulation of 2,4-D degradation may involve specific adaptation and selection mechanisms (as in the case with samples previously enriched with 2,4-D or its structural analogues--aromatic monomers, ethanol) as well as non-specific mechanisms. The extent of mineralization of 2,4-D was not affected by soil pretreatment, about 1/3 of introduced radioactive carbon being invariably transformed to 14CO2.
- MeSH
- ethanol farmakologie MeSH
- glukosa farmakologie MeSH
- hydroxybenzoáty farmakologie MeSH
- kyselina 2,4-dichlorfenoxyoctová metabolismus MeSH
- kyselina vanilová farmakologie MeSH
- oxid uhličitý metabolismus MeSH
- půdní mikrobiologie * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ethanol MeSH
- glukosa MeSH
- hydroxybenzoáty MeSH
- kyselina 2,4-dichlorfenoxyoctová MeSH
- kyselina vanilová MeSH
- oxid uhličitý MeSH
- protocatechuic acid MeSH Prohlížeč
Decomposition of preparations of various fractions of fulvic acids in pure cultures of bacteria and in the soil was investigated. In the soils enriched with fulvic acids the amount of bacteria increased, oxygen consumption and formation of carbon dioxide followed a typical sigmoid curve. The above measurements indicated that mineralization occurred after a very short or negligible lag phase. During the decomposition of fulvic acids the ability of microorganisms to oxidize aromatic compounds, e.g. vanillic and p-hydroxybenzoic acid, increased. The presence of aromatic structures in the used preparations of fulvic acids was demonstrated on the basis of their IR spectra and according to the results of chromatographic analyses of their hydrolysates. The results indicated a relationship between metabolism of fulvic acids and aromatic compounds. In samples of the soil preincubated with glucose with fulvic acids decomposed more rapidly than in untreated samples.
- MeSH
- Bacteria metabolismus MeSH
- benzopyrany metabolismus MeSH
- biodegradace MeSH
- hydroxybenzoáty metabolismus MeSH
- kyselina vanilová metabolismus MeSH
- oxid uhličitý biosyntéza MeSH
- půdní mikrobiologie * MeSH
- spotřeba kyslíku MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzopyrany MeSH
- hydroxybenzoáty MeSH
- kyselina vanilová MeSH
- oxid uhličitý MeSH
- MeSH
- acetamidy farmakologie MeSH
- Actinomycetales metabolismus MeSH
- aktivní transport MeSH
- anaerobióza MeSH
- azidy farmakologie MeSH
- benzoáty metabolismus MeSH
- buněčná membrána metabolismus MeSH
- chloramfenikol farmakologie MeSH
- dinitrofenoly farmakologie MeSH
- frakcionace buněk MeSH
- glukosa farmakologie MeSH
- jod MeSH
- kyselina vanilová metabolismus MeSH
- manometrie MeSH
- oxid uhličitý biosyntéza MeSH
- oxidace-redukce MeSH
- radioizotopy uhlíku MeSH
- spotřeba kyslíku MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- acetamidy MeSH
- azidy MeSH
- benzoáty MeSH
- chloramfenikol MeSH
- dinitrofenoly MeSH
- glukosa MeSH
- jod MeSH
- kyselina vanilová MeSH
- oxid uhličitý MeSH
- radioizotopy uhlíku MeSH
- Klíčová slova
- NICOTINIC ACID ISOMERS/determination *, VANILLIC ACID *,
- MeSH
- benzaldehydy * MeSH
- isomerie * MeSH
- kyselina vanilová * MeSH
- kyseliny nikotinové analýza MeSH
- niacin * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzaldehydy * MeSH
- kyselina vanilová * MeSH
- kyseliny nikotinové MeSH
- niacin * MeSH
- vanillin MeSH Prohlížeč
