Caffeic acid belongs to the polyphenol compounds we consume daily, often in the form of coffee. Even though it is less explored than caffeic acid phenethyl ester, it still has many positive effects on human health. Caffeic acid can affect cancer, diabetes, atherosclerosis, Alzheimer's disease, or bacterial and viral infections. This review focuses on the molecular mechanisms of how caffeic acid achieves its effects.

• Klíčová slova
• Alzheimer’s disease, atherosclerosis, caffeic acid, cancer, diabetes, obesity,
• MeSH
• fenethylalkohol * farmakologie   MeSH
• kyseliny kávové * farmakologie   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• caffeic acid MeSH Prohlížeč
• fenethylalkohol * MeSH
• kyseliny kávové * MeSH

Salinity stress adversely affects plant growth by disrupting water uptake, inducing ion toxicity, initiating osmotic stress, impairing growth, leaf scorching, and reducing crop yield. To mitigate this issue, the application of kaempferol (KP), caffeic acid (CA), and plant growth-promoting rhizobacteria (PGPR) emerges as a promising technology. Kaempferol, a flavonoid, protects plants from oxidative stress, while caffeic acid, a plant-derived compound, promotes growth by regulating physiological processes. PGPR enhances plant health and productivity through growth promotion, nutrient uptake, and stress mitigation, providing a sustainable solution. However, combining these compounds against drought requires further scientific justification. That's why the current study was conducted using 4 treatments, i.e., 0, 20 µM KP, 30 μM CA, and 20 µM KP + 30 μM CA without and with PGPR (Bacillus altitudinis). There were 4 replications following a completely randomized design. Results showed that 20 µM KP + 30 μM CA with PGPR caused significant enhancement in potato stem length (14.32%), shoot root, and leaf dry weight (16.52%, 11.04%, 67.23%), than the control. The enrichment in potato chlorophyll a, b, and total (31.86%, 46.05%, and 35.52%) was observed over the control, validating the potential of 20 µM KP + 30 μM CA + PGPR. Enhancement in shoot N, P, K, and Ca concentration validated the effective functioning of 20 µM KP + 30 μM CA with PGPR evaluated to control. In conclusion, 20 µM KP + 30 μM CA with PGPR is the recommended amendment to alleviate salinity stress in potatoes.

• Klíčová slova
• Antioxidant, Caffeic acid, Flavonoid, Growth attributes, Salinity stress,
• MeSH
• chlorofyl metabolismus   MeSH
• kempferoly * MeSH
• kořeny rostlin růst a vývoj   účinky léků   MeSH
• kyseliny kávové * MeSH
• listy rostlin růst a vývoj   účinky léků   metabolismus   MeSH
• Solanum tuberosum * růst a vývoj   účinky léků   metabolismus   MeSH
• solný stres * účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid MeSH Prohlížeč
• chlorofyl MeSH
• kaempferol MeSH Prohlížeč
• kempferoly * MeSH
• kyseliny kávové * MeSH

Dental caries is a biofilm-related disease, widely perceived to be caused by oral ecological imbalance when cariogenic/aciduric bacteria obtain an ecological advantage. Compared with planktonic bacteria, dental plaques are difficult to remove under extracellular polymeric substance protection. In this study, the effect of caffeic acid phenethyl ester (CAPE) on a preformed cariogenic multi-species biofilm was evaluated, which was comprised of cariogenic bacteria (Streptococcus mutans), commensal bacteria (Streptococcus gordonii), and a pioneer colonizer (Actinomyces naeslundii). Our result revealed that treatment with 0.08 mg/mL CAPE reduced live S. mutans in the preformed multi-species biofilm while not significantly changing the quantification of live S. gordonii. CAPE significantly reduced the production of lactic acid, extracellular polysaccharide, and extracellular DNA and made the biofilm looser. Moreover, CAPE could promote the H2O2 production of S. gordonii and inhibit the expression of SMU.150 encoding mutacin to modulate the interaction among species in biofilms. Overall, our results suggested that CAPE could inhibit the cariogenic properties and change the microbial composition of the multi-species biofilms, indicating its application potential in dental caries prevention and management.

• Klíčová slova
• Antibiofilm, Caffeic acid phenethyl ester, Dental caries, Multi-species biofilms, Streptococcus mutans,
• MeSH
• biofilmy MeSH
• lidé MeSH
• matrix extracelulárních polymerních látek metabolismus   MeSH
• peroxid vodíku * metabolismus   MeSH
• Streptococcus mutans metabolismus   MeSH
• zubní kaz * prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid phenethyl ester MeSH Prohlížeč
• peroxid vodíku * MeSH

We investigated a possible enhancement of all-trans retinoic acid (ATRA)-induced differentiation of HL-60 human myeloid leukemia cells by caffeic acid (CA), a widely distributed plant phenolic compound. Our results showed that CA, in the concentration of 13 or 52 micro M, had no or minimal influence on cell differentiation, whereas the differentiating activity of ATRA was potentiated by CA treatment. We proved, using flow cytometric detection of the CD66b surface molecule, a synergistic effect of CA: at day 10, 18.3% of CD66b-positive cells were detected after treatment with ATRA only, and 33% when CA and ATRA were combined together. NBT-assay confirmed that this additive effect of CA on ATRA-induced differentiation. Proliferating activity as assessed by MTT-assay was generally not affected by CA at given concentrations. However, cell proliferation was significantly reduced by 52 micro M CA at 96-h intervals. This effect was markedly enhanced when CA, at both concentrations, and ATRA were combined. The possibility to enhance the differentiation potential of ATRA by CA may improve outcomes in the therapy of acute promyelocytic leukemia.

• MeSH
• akutní promyelocytární leukemie farmakoterapie   MeSH
• antigeny nádorové biosyntéza   MeSH
• barvicí látky farmakologie   MeSH
• buněčná diferenciace MeSH
• časové faktory MeSH
• CD antigeny MeSH
• chemické modely MeSH
• GPI-vázané proteiny MeSH
• HL-60 buňky MeSH
• imunofenotypizace MeSH
• kyseliny kávové aplikace a dávkování   chemie   MeSH
• lidé MeSH
• molekuly buněčné adheze biosyntéza   MeSH
• proliferace buněk MeSH
• protokoly antitumorózní kombinované chemoterapie terapeutické užití   MeSH
• průtoková cytometrie MeSH
• synergismus léků * MeSH
• tetrazoliové soli farmakologie   MeSH
• thiazoly farmakologie   MeSH
• tretinoin aplikace a dávkování   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny nádorové MeSH
• barvicí látky MeSH
• caffeic acid MeSH Prohlížeč
• CD antigeny MeSH
• CEACAM8 protein, human MeSH Prohlížeč
• GPI-vázané proteiny MeSH
• kyseliny kávové MeSH
• molekuly buněčné adheze MeSH
• tetrazoliové soli MeSH
• thiazoly MeSH
• thiazolyl blue MeSH Prohlížeč
• tretinoin MeSH

Warfarin is a commonly used anticoagulant drug and is a derivate of coumarin. Cytochrome P450 2C9 (CYP2C9) plays the key role in transformation of coumarin and thus, influences determination of warfarin dosage. A number of factors including dietary compounds such as sesamin, caffeic acid and ferulic acids can regulate the activity of CYP2C9. The present study tested the hypothesis that sesamin, episesamin, caffeic acid and ferulic acid decreases the rate of warfarin 7-hydroxylation via inhibition of hepatic CYP2C9. The experiments were conducted on hepatic microsomes from human donors. It was demonstrated that the rate of 7-hydroxylation of warfarin was significantly decreased in the presence of sesamin in the range of concentrations from 5 to 500 nM, and was not affected by episesamin, caffeic acid and ferulic acid in the same range of concentrations. The kinetic analysis indicated non-competitive type of inhibition by sesamin with Ki = 202 ± 18 nM. In conclusion, the results of our in vitro study revealed that sesamin was able to inhibit formation of a major metabolite of warfarin, 7-hydroxywarfarin. The potentially negative consequences of the consumption of high amounts of sesamin-containing food or dietary supplements in warfarin-treated patients need to be further studied.

• Klíčová slova
• Cytochrome P450, Detoxification, Food-drug interactions, In vitro, S-7-hydroxywarfarin,
• MeSH
• antikoagulancia metabolismus   MeSH
• dioxoly chemie   farmakologie   MeSH
• hydroxylace MeSH
• inhibiční koncentrace 50 MeSH
• jaterní mikrozomy metabolismus   MeSH
• kinetika MeSH
• kyseliny kávové farmakologie   MeSH
• kyseliny kumarové farmakologie   MeSH
• lidé MeSH
• lignany chemie   farmakologie   MeSH
• potraviny MeSH
• potravní doplňky MeSH
• warfarin metabolismus   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antikoagulancia MeSH
• caffeic acid MeSH Prohlížeč
• dioxoly MeSH
• ferulic acid MeSH Prohlížeč
• kyseliny kávové MeSH
• kyseliny kumarové MeSH
• lignany MeSH
• sesamin MeSH Prohlížeč
• warfarin MeSH

Possible UVC-protective properties of CA, a plant phenolic compound with antioxidant activity, were investigated on human KF1 diploid fibroblast and A431 epidermoid carcinoma cell lines. Cell populations, untreated and treated by antioxidants (CA and alpha-tocopherol), were irradiated by UVC at the wavelength of 254 nm and their proliferation activity was determined by the MTT assay. The results show a strong protective effect of CA at both concentrations used (55.5 and 166.5 microM): a significant increase of proliferation activity after UVC irradiation was detected in both cell populations growing in the presence of CA in comparison with cells in DMEM only. The described protective effect of CA was more obvious in transformed cells than in normal diploid cells. This protective ability is probably based on the antioxidant and scavenging activities of CA, which seems to be more efficient than alpha-tocopherol in protection against the cytotoxic effect caused by UVC irradiation.

• MeSH
• buněčné dělení účinky léků   účinky záření   MeSH
• buněčné linie MeSH
• kůže cytologie   účinky léků   patologie   účinky záření   MeSH
• kyseliny kávové farmakologie   MeSH
• lidé MeSH
• nádorová transformace buněk účinky léků   patologie   účinky záření   MeSH
• ochranné látky farmakologie   MeSH
• ultrafialové záření * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• caffeic acid MeSH Prohlížeč
• kyseliny kávové MeSH
• ochranné látky MeSH

Potatoes (Solanum tuberosum L.) are a significant food crop cultivated around the world. Caffeic acid (CA) can enhance plant growth by promoting antioxidant activity and stimulating root development, contributing to overall plant health and vigor. Cobalt sulfate (CoSO4) boosts plant growth by promoting nitrogen (N) fixation, healthier root development, and chlorophyll synthesis, enhancing photosynthesis and overall plant health. Nanoparticle-coated urea (NPCU) improves nutrient uptake, promoting plant growth efficiency and reducing environmental impact. This study investigates the effects of combining CA, CoSO4, and NPCU as amendments on potatoes with and without NPCU. Four treatments, control, 20 μM CA, 0.15 mg/L CoSO4, and 20 μM CA + 0.15 mg/L CoSO4 with and without NPCU, were applied in four replications using a completely randomized design. Results demonstrate that the combination of CA + CoSO4 with NPCU led to an increase in potato stem length (~ 6%), shoot dry weight (~ 15%), root dry weight (~ 9%), and leaf dry weight (~ 49%) compared to the control in nutrient stress. There was a significant rise in chlorophyll a (~ 27%), chlorophyll b (~ 37%), and total chlorophyll (~ 28%) over the control under nutrient stress also showed the potential of CA + CoSO4 with NPCU. In conclusion, the findings suggest that applying CA + CoSO4 with NPCU is a strategy for alleviating potato nutrient stress.

• Klíčová slova
• Solanum tuberosum L., Chlorophyll content, Growth attributes, N-fertilizer,
• MeSH
• chlorofyl metabolismus   MeSH
• fotosyntéza účinky léků   MeSH
• kobalt farmakologie   chemie   MeSH
• kořeny rostlin účinky léků   růst a vývoj   MeSH
• kyseliny kávové * farmakologie   chemie   MeSH
• listy rostlin účinky léků   MeSH
• močovina * farmakologie   MeSH
• nanočástice * chemie   MeSH
• Solanum tuberosum * účinky léků   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid MeSH Prohlížeč
• chlorofyl MeSH
• kobalt MeSH
• kyseliny kávové * MeSH
• močovina * MeSH

Severe burn injuries cause functional impairment in distant internal organs. Although this mechanism is not clear, it is possible that free radical toxicity plays an important role. Research in animals and clinical studies have shown that there is a close relationship between a lipid peroxidative reaction and secondary pathological changes following thermal injury. It has been demonstrated that antioxidant treatment prevents oxidative tissue damage associated with thermal trauma. This study was designed to determine the possible protective effect of caffeic acid phenethyl ester (CAPE) treatment against oxidative damage in the kidney and lung induced by thermal injury. Rats were decapitated either 1, 3 or 7 days after burn injury. CAPE was administered intraperitoneally immediately after thermal injury. Kidney and lung tissues were taken for the determination of malondialdehyde (MDA) level, myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD) and xanthine oxidase (XO) activities. Severe skin thermal injury caused a significant decrease in SOD and CAT activities, as well as significant increases in MDA level, XO and MPO activities in tissues during the postburn period. Treatment of rats with CAPE (10 micromol/kg) significantly elevated the decreased SOD and CAT activities, while it decreased MDA levels and MPO as well as XO activity.

• MeSH
• fenethylalkohol aplikace a dávkování   analogy a deriváty   MeSH
• injekce intraperitoneální MeSH
• krysa rodu Rattus MeSH
• kyseliny kávové aplikace a dávkování   MeSH
• malondialdehyd metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• multiorgánové selhání farmakoterapie   etiologie   metabolismus   MeSH
• nemoci ledvin farmakoterapie   etiologie   metabolismus   MeSH
• oxidoreduktasy metabolismus   MeSH
• plicní nemoci farmakoterapie   etiologie   metabolismus   MeSH
• popálení komplikace   metabolismus   MeSH
• potkani Wistar MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid phenethyl ester MeSH Prohlížeč
• fenethylalkohol MeSH
• kyseliny kávové MeSH
• malondialdehyd MeSH
• oxidoreduktasy MeSH

Smallanthus sonchifolius (yacon) is an edible tuberous Andean shrub that has been included in the diet of indigenous people since before recorded history. The nutraceutical and medicinal properties of yacon are widely recognized, especially for the improvement of hyperglycemic disorders. However, the chemical diversity of the main bioactive series of caffeic acid esters has not been explored in detail. In this metabolomics study, we applied the latest tools to facilitate the targeted isolation of new caffeic acid esters. Using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), we analyzed extracts from different organs (roots, vascular tissues of the stems, stem epidermis, leaves, bracts, and ray flowers) and followed a feature-based molecular networking approach to characterize the structural diversity of caffeic acid esters and recognize new compounds. The analysis identified three potentially new metabolites, one of them confirmed by isolation and full spectroscopic/spectrometric assignment using nuclear magnetic resonance (NMR), high-resolution mass spectrometry (HRMS), and MS/MS. This metabolite (5-O-caffeoyl-2,7-anhydro-d-glycero-β-d-galacto-oct-2-ulopyranosonic acid), along with eight known caffeic acid esters, was isolated from the roots and stems. Furthermore, based on detailed tandem MS analyses, we suggest that the two isomeric monocaffeoyl-2,7-anhydro-2-octulopyranosonic acids found in yacon can be reliably distinguished based on their characteristic MS2 and MS3 spectra. The outcome of the current study confirms the utility of feature-based molecular networking as a tool for targeted isolation of previously undescribed metabolites and reveals the full diversity of potentially bioactive metabolites from S. sonchifolius.

• Klíčová slova
• caffeic acid esters, chlorogenic acids, metabolomics, molecular networking,
• Publikační typ
• časopisecké články MeSH

The chemoprotective effects of caffeic (CA), chlorogenic (CHA) and rosmarinic (RA) acids were tested against the toxicity of doxorubicin (DOX) in neonatal rat cardiomyocytes and the iron-dependent DOX induced lipid peroxidation of heart membranes, mitochondria and microsomes. The protectivity of these acids was compared with dexrazoxan, used as an adjuvant during DOX chemotherapy. The cytoprotective effects were assessed by enzyme (LDH and ASAT) and troponin I leakage, secondly by intracellular ATP content. All hydroxycinnamic acids proved non-cytotoxic, and they stabilized both membranes and the energetic status of cardiomyocytes. After preincubation of cardiomyocytes with the test compounds (100, 200 microm; 1 h) the cardiomyocytes were treated with the toxic agent, DOX (100 microm; 8 h). The test compounds protected cardiomyocytes against DOX induced oxidative stress (RA > CHA > or = CA) on all monitored parameters. Substantial preservation of monolayer integrity of the cardiomyocytes by test compounds was also found microscopically. All the acids were more effective in the assays used than dexrazoxan. RA showed the most effective cytoprotectivity. All the acids significantly reduced the iron-dependent DOX induced lipid peroxidation of heart membranes, although of the test compounds, CHA was found to be the most effective (IC(50) = 8.04 +/- 0.74/6.87 +/- 0.52 micro m for microsomes/mitochondria).

• MeSH
• cinnamáty aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• depsidy MeSH
• doxorubicin MeSH
• fytoterapie * MeSH
• inhibiční koncentrace 50 MeSH
• kardiomyocyty účinky léků   enzymologie   MeSH
• krysa rodu Rattus MeSH
• kyselina chlorogenová aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• kyselina rozmarýnová MeSH
• kyseliny kávové aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• léčivé rostliny * MeSH
• mikrozomy účinky léků   MeSH
• myokard cytologie   enzymologie   MeSH
• nemoci srdce chemicky indukované   patologie   prevence a kontrola   MeSH
• ochranné látky aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• peroxidace lipidů účinky léků   MeSH
• potkani Wistar MeSH
• rostlinné extrakty aplikace a dávkování   farmakologie   terapeutické užití   MeSH
• srdeční mitochondrie účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cinnamáty MeSH
• depsidy MeSH
• doxorubicin MeSH
• kyselina chlorogenová MeSH
• kyseliny kávové MeSH
• ochranné látky MeSH
• rostlinné extrakty MeSH