Dental caries is a biofilm-related disease, widely perceived to be caused by oral ecological imbalance when cariogenic/aciduric bacteria obtain an ecological advantage. Compared with planktonic bacteria, dental plaques are difficult to remove under extracellular polymeric substance protection. In this study, the effect of caffeic acid phenethyl ester (CAPE) on a preformed cariogenic multi-species biofilm was evaluated, which was comprised of cariogenic bacteria (Streptococcus mutans), commensal bacteria (Streptococcus gordonii), and a pioneer colonizer (Actinomyces naeslundii). Our result revealed that treatment with 0.08 mg/mL CAPE reduced live S. mutans in the preformed multi-species biofilm while not significantly changing the quantification of live S. gordonii. CAPE significantly reduced the production of lactic acid, extracellular polysaccharide, and extracellular DNA and made the biofilm looser. Moreover, CAPE could promote the H2O2 production of S. gordonii and inhibit the expression of SMU.150 encoding mutacin to modulate the interaction among species in biofilms. Overall, our results suggested that CAPE could inhibit the cariogenic properties and change the microbial composition of the multi-species biofilms, indicating its application potential in dental caries prevention and management.

• Klíčová slova
• Antibiofilm, Caffeic acid phenethyl ester, Dental caries, Multi-species biofilms, Streptococcus mutans,
• MeSH
• biofilmy MeSH
• lidé MeSH
• matrix extracelulárních polymerních látek metabolismus   MeSH
• peroxid vodíku * metabolismus   MeSH
• Streptococcus mutans metabolismus   MeSH
• zubní kaz * prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid phenethyl ester MeSH Prohlížeč
• peroxid vodíku * MeSH

Severe burn injuries cause functional impairment in distant internal organs. Although this mechanism is not clear, it is possible that free radical toxicity plays an important role. Research in animals and clinical studies have shown that there is a close relationship between a lipid peroxidative reaction and secondary pathological changes following thermal injury. It has been demonstrated that antioxidant treatment prevents oxidative tissue damage associated with thermal trauma. This study was designed to determine the possible protective effect of caffeic acid phenethyl ester (CAPE) treatment against oxidative damage in the kidney and lung induced by thermal injury. Rats were decapitated either 1, 3 or 7 days after burn injury. CAPE was administered intraperitoneally immediately after thermal injury. Kidney and lung tissues were taken for the determination of malondialdehyde (MDA) level, myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD) and xanthine oxidase (XO) activities. Severe skin thermal injury caused a significant decrease in SOD and CAT activities, as well as significant increases in MDA level, XO and MPO activities in tissues during the postburn period. Treatment of rats with CAPE (10 micromol/kg) significantly elevated the decreased SOD and CAT activities, while it decreased MDA levels and MPO as well as XO activity.

• MeSH
• fenethylalkohol aplikace a dávkování   analogy a deriváty   MeSH
• injekce intraperitoneální MeSH
• krysa rodu Rattus MeSH
• kyseliny kávové aplikace a dávkování   MeSH
• malondialdehyd metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• multiorgánové selhání farmakoterapie   etiologie   metabolismus   MeSH
• nemoci ledvin farmakoterapie   etiologie   metabolismus   MeSH
• oxidoreduktasy metabolismus   MeSH
• plicní nemoci farmakoterapie   etiologie   metabolismus   MeSH
• popálení komplikace   metabolismus   MeSH
• potkani Wistar MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• caffeic acid phenethyl ester MeSH Prohlížeč
• fenethylalkohol MeSH
• kyseliny kávové MeSH
• malondialdehyd MeSH
• oxidoreduktasy MeSH

Caffeic acid belongs to the polyphenol compounds we consume daily, often in the form of coffee. Even though it is less explored than caffeic acid phenethyl ester, it still has many positive effects on human health. Caffeic acid can affect cancer, diabetes, atherosclerosis, Alzheimer's disease, or bacterial and viral infections. This review focuses on the molecular mechanisms of how caffeic acid achieves its effects.

• Klíčová slova
• Alzheimer’s disease, atherosclerosis, caffeic acid, cancer, diabetes, obesity,
• MeSH
• fenethylalkohol * farmakologie   MeSH
• kyseliny kávové * farmakologie   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• caffeic acid MeSH Prohlížeč
• fenethylalkohol * MeSH
• kyseliny kávové * MeSH

From the chloroform part of Ligustrum delavayanum Hariot, leaves five constituents were isolated: tyrosol, esculetine, kempferol-7-O-rhamnoside, quercetine-3-O-glucoside, and quercetine. Further six constituents (probably malvidine, two secoiridoid glycosides, caffeic acid monosaccharide ester, quercetine monoglycoside (no hyperoside), and quercetine diglycoside (no rutinoside)) were isolated from the methanolic part of above mentioned drug.

• MeSH
• fenethylalkohol analogy a deriváty   izolace a purifikace   MeSH
• flavonoidy izolace a purifikace   MeSH
• kempferoly * MeSH
• léčivé rostliny chemie   MeSH
• listy rostlin chemie   MeSH
• Oleaceae chemie   MeSH
• quercetin analogy a deriváty   izolace a purifikace   MeSH
• umbeliferony izolace a purifikace   MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• 4-hydroxyphenylethanol MeSH Prohlížeč
• esculetin MeSH Prohlížeč
• fenethylalkohol MeSH
• flavonoidy MeSH
• isoquercitrin MeSH Prohlížeč
• kaempferol rhamnorobinoside MeSH Prohlížeč
• kempferoly * MeSH
• quercetin MeSH
• umbeliferony MeSH