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MeSH: kyselina gallová-chemie

3 záznamů v PubMed Filtry

Článek

Ultrasound treatment modified the functional mode of gallic acid on properties of fish myofibrillar protein

Pan, Jinfeng
Autor Pan, Jinfeng National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, České Budějovice 370 05, Czech Republic. Electronic address: pjf613@163.com
Lian, Hongliang
Autor Lian, Hongliang National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Jia, Hui
Autor Jia, Hui University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, České Budějovice 370 05, Czech Republic
Li, Shengjie
Autor Li, Shengjie National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Hao, Ruoyi
Autor Hao, Ruoyi University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, České Budějovice 370 05, Czech Republic
Wang, Yujie
Autor Wang, Yujie National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Zhang, Xuening
Autor Zhang, Xuening National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Dong, Xiuping
Autor Dong, Xiuping National Engineering Research Center for Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, College of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China

Food chemistry. 2020 Aug 01 ; 320 () : 126637. [epub] 20200318

Food Chem
ISSN 1873-7072 | 0308-8146
Zdroj

Effects of 0, 1, 5, 25 and 125 μmol/g gallic acid (GA) without or with ultrasound treatment (20 kHz, 400 w, 5 min) (NU or U groups) on properties of Japanese seerfish myofibrillar protein (MP) were studied. After sonication, tryptophan fluorescence decreased while surface hydrophobicity, free amine and SH content (not U125) and solubility increased. After heating, NU125 showed the heaviest polymers among NU groups, but U5 exhibited the strongest while U125 showed the weakest polymers in U groups. Storage modulus (G') of NU groups showed a dose-dependent style, but for U groups, U5 had the highest G' while U125 had the lowest G'. Mass analysis confirmed the formation of Cys-GA-Cys and Lys-GA-Lys polymers in U125. Thus, ultrasound promoted structural unfolding and reactive groups exposure, producing GA quinone by triggering OH·. These together led to the G' improvement by low dose GA but deterioration by high does GA.

Klíčová slova
2,4,6-Trinitrobenzenesulfonic acid (PubChem CID: 11045), 5,5′-Dithiobis-(2-nitrobenzoic acid) (PubChem CID: 6254), Amino-quinone adduct, Bromophenol blue (PubChem CID: 8272), Gallic acid, Gallic acid (PubChem CID: 370), Hydroxyl radical, Maleic acid (PubChem CID: 444266), Myofibrillar protein, N-Ethylmaleimide (PubChem CID: 4362), Rheological properties, Sodium dodecyl sulfate (PubChem CID: 3423265), Thiol-quinone adduct, Ultrasound, l-Leucine (PubChem CID: 6106), β-Mercaptoethanol (PubChem CID: 1567),
MeSH
hydrofobní a hydrofilní interakce MeSH
kyselina gallová chemie MeSH
rybí proteiny chemie MeSH
ultrazvuk MeSH
velikost částic MeSH
zvířata MeSH
Check Tag
zvířata MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
kyselina gallová MeSH
rybí proteiny MeSH

Článek

LC-MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model

Journal of pharmaceutical and biomedical analysis. 2013 Dec ; 86 () : 135-42. [epub] 20130814

J Pharm Biomed Anal
ISSN 1873-264X | 0731-7085
Zdroj

Galloyl esters of quercetin and taxifolin have been recently prepared semisynthetically as part of work towards modifying the solubility and modulating the biological activity of these natural flavonoids. In this paper we focused on the liquid chromatography-mass spectrometry (LC-MS) profiling of metabolites of 3-O-galloylquercetin and 7-O-galloyltaxifolin using human hepatocytes as the in vitro cell model. A subtoxic concentration (50μM) was used for both compounds and the formation of metabolites was monitored for 2h in hepatocytes and cultivation medium separately. Using negative electrospray ionization-quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS), we identified different biotransformation patterns for the studied compounds. 3-O-Galloylquercetin is metabolized directly to glucuronides and methyl derivatives. In contrast, 7-O-galloyltaxifolin is oxidized to 7-O-galloylquercetin or cleaved to taxifolin, and consequently the products formed are sulfated or glucuronidated. The oxidative biotransformation of 3-O-galloylquercetin and 7-O-galloyltaxifolin is also accompanied by ester bond cleavage presumably by cellular enzymes (esterases) in a nonspecific manner. Our results provide fundamental insights into the biotransformation of monogalloyl esters of flavonoids and can be applied in investigations of the pharmaceutical potential of other galloylated polyphenolic substances.

Klíčová slova
3-O-galloylquercetin, 3-O-galloylquercetin glucuronide, 3-O-galloylquercetin methyl derivative, 3GQ, 3GQG, 3GQM, 7-O-galloylquercetin, 7-O-galloyltaxifolin, 7GQ, 7GT, Cytotoxicity, ESI-QqTOF MS, GA, HPLC, LC–MS, Mass spectrometry, Metabolic transformation, PHE, Quercetin-3-O-gallate, SIM, TG, TS, Taxifolin-7-O-gallate, electrospray ionization-quadrupole time-of-flight mass spectrometry, gallic acid (gallate), high-performance liquid chromatography, liquid chromatography–mass spectrometry, phenyl, selected-ion monitoring, taxifolin glucuronide, taxifolin sulfate,
MeSH
biotransformace MeSH
buňky NIH 3T3 MeSH
chromatografie kapalinová metody MeSH
estery MeSH
hepatocyty účinky léků metabolismus MeSH
hmotnostní spektrometrie metody MeSH
kultivované buňky MeSH
kyselina gallová chemie farmakokinetika toxicita MeSH
lidé středního věku MeSH
lidé MeSH
mladiství MeSH
myši inbrední BALB C MeSH
myši MeSH
quercetin analogy a deriváty chemie farmakokinetika toxicita MeSH
tandemová hmotnostní spektrometrie metody MeSH
zvířata MeSH
Check Tag
lidé středního věku MeSH
lidé MeSH
mladiství MeSH
mužské pohlaví MeSH
myši MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
estery MeSH
kyselina gallová MeSH
quercetin MeSH
quercitrin MeSH Prohlížeč
taxifolin MeSH Prohlížeč

Článek

Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases

Journal of chromatography. A. 2008 Jun 27 ; 1195 (1-2) : 52-9. [epub] 20080503

J Chromatogr A
ISSN 0021-9673
Zdroj

Complete separation of aglycones and glucosides of selected isoflavones (genistin, genistein, daidzin, daidzein, glycitin, glycitein, ononin, sissotrin, formononetin, and biochanin A) was possible in 1.5 min using an ultrahigh-pressure liquid chromatography (U-HPLC) on a different particular chemically modified stationary phases with a particle size under 2 microm. In addition, selected separation conditions for simultaneous determination of isoflavones together with a group of phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, and sinapic acid) allowed separation of all 19 compounds in 1.9 min. Separations were conducted on a non-polar reversed phase (C(18)) and also on more polar phases with cyanopropyl or phenyl groups using a gradient elution with a mobile phase consisting of 0.3% aqueous acetic acid and methanol. Chromatographic peaks were characterised using parameters such as resolution, symmetry, selectivity, etc. Individual substances were identified and quantified using UV-vis diode array detector at wavelength 270 nm. Limits of detection (3S/N) were in the range 200-400 pg ml(-1). Proposed U-HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials (Trifolium pratense, Glycine max, Pisum sativum and Ononis spinosa) after acid hydrolysis of the samples and modified Soxhlet extraction.

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