Complete separation of aglycones and glucosides of selected isoflavones (genistin, genistein, daidzin, daidzein, glycitin, glycitein, ononin, sissotrin, formononetin, and biochanin A) was possible in 1.5 min using an ultrahigh-pressure liquid chromatography (U-HPLC) on a different particular chemically modified stationary phases with a particle size under 2 microm. In addition, selected separation conditions for simultaneous determination of isoflavones together with a group of phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, and sinapic acid) allowed separation of all 19 compounds in 1.9 min. Separations were conducted on a non-polar reversed phase (C(18)) and also on more polar phases with cyanopropyl or phenyl groups using a gradient elution with a mobile phase consisting of 0.3% aqueous acetic acid and methanol. Chromatographic peaks were characterised using parameters such as resolution, symmetry, selectivity, etc. Individual substances were identified and quantified using UV-vis diode array detector at wavelength 270 nm. Limits of detection (3S/N) were in the range 200-400 pg ml(-1). Proposed U-HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials (Trifolium pratense, Glycine max, Pisum sativum and Ononis spinosa) after acid hydrolysis of the samples and modified Soxhlet extraction.
- MeSH
- genistein chemie izolace a purifikace MeSH
- Glycine max chemie MeSH
- hrách setý chemie MeSH
- hydroxybenzoáty chemie izolace a purifikace MeSH
- isoflavony chemie izolace a purifikace MeSH
- kyselina gallová analogy a deriváty chemie izolace a purifikace MeSH
- kyselina vanilová chemie izolace a purifikace MeSH
- kyseliny kávové chemie izolace a purifikace MeSH
- kyseliny kumarové chemie izolace a purifikace MeSH
- molekulární struktura MeSH
- propionáty MeSH
- rostlinné extrakty chemie izolace a purifikace MeSH
- Trifolium chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biochanin A MeSH Prohlížeč
- caffeic acid MeSH Prohlížeč
- daidzein MeSH Prohlížeč
- daidzin MeSH Prohlížeč
- ferulic acid MeSH Prohlížeč
- formononetin MeSH Prohlížeč
- genistein MeSH
- genistin MeSH Prohlížeč
- glycitein MeSH Prohlížeč
- glycitin MeSH Prohlížeč
- hydroxybenzoáty MeSH
- isoflavony MeSH
- kyselina gallová MeSH
- kyselina vanilová MeSH
- kyseliny kávové MeSH
- kyseliny kumarové MeSH
- p-coumaric acid MeSH Prohlížeč
- phenolic acid MeSH Prohlížeč
- propionáty MeSH
- protocatechuic acid MeSH Prohlížeč
- rostlinné extrakty MeSH
- sinapinic acid MeSH Prohlížeč
- syringic acid MeSH Prohlížeč
