Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18501366
DOI
10.1016/j.chroma.2008.04.069
PII: S0021-9673(08)00752-8
Knihovny.cz E-resources
- MeSH
- Genistein chemistry isolation & purification MeSH
- Glycine max chemistry MeSH
- Pisum sativum chemistry MeSH
- Hydroxybenzoates chemistry isolation & purification MeSH
- Isoflavones chemistry isolation & purification MeSH
- Gallic Acid analogs & derivatives chemistry isolation & purification MeSH
- Vanillic Acid chemistry isolation & purification MeSH
- Caffeic Acids chemistry isolation & purification MeSH
- Coumaric Acids chemistry isolation & purification MeSH
- Molecular Structure MeSH
- Propionates MeSH
- Plant Extracts chemistry isolation & purification MeSH
- Trifolium chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- biochanin A MeSH Browser
- caffeic acid MeSH Browser
- daidzein MeSH Browser
- daidzin MeSH Browser
- ferulic acid MeSH Browser
- formononetin MeSH Browser
- Genistein MeSH
- genistin MeSH Browser
- glycitein MeSH Browser
- glycitin MeSH Browser
- Hydroxybenzoates MeSH
- Isoflavones MeSH
- Gallic Acid MeSH
- Vanillic Acid MeSH
- Caffeic Acids MeSH
- Coumaric Acids MeSH
- p-coumaric acid MeSH Browser
- phenolic acid MeSH Browser
- Propionates MeSH
- protocatechuic acid MeSH Browser
- Plant Extracts MeSH
- sinapinic acid MeSH Browser
- syringic acid MeSH Browser
Complete separation of aglycones and glucosides of selected isoflavones (genistin, genistein, daidzin, daidzein, glycitin, glycitein, ononin, sissotrin, formononetin, and biochanin A) was possible in 1.5 min using an ultrahigh-pressure liquid chromatography (U-HPLC) on a different particular chemically modified stationary phases with a particle size under 2 microm. In addition, selected separation conditions for simultaneous determination of isoflavones together with a group of phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, ferulic, and sinapic acid) allowed separation of all 19 compounds in 1.9 min. Separations were conducted on a non-polar reversed phase (C(18)) and also on more polar phases with cyanopropyl or phenyl groups using a gradient elution with a mobile phase consisting of 0.3% aqueous acetic acid and methanol. Chromatographic peaks were characterised using parameters such as resolution, symmetry, selectivity, etc. Individual substances were identified and quantified using UV-vis diode array detector at wavelength 270 nm. Limits of detection (3S/N) were in the range 200-400 pg ml(-1). Proposed U-HPLC technique was used for separation of isoflavones and phenolic acids in samples of plant materials (Trifolium pratense, Glycine max, Pisum sativum and Ononis spinosa) after acid hydrolysis of the samples and modified Soxhlet extraction.
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