Claviceps paspali FA produced high concentrations of alkaloid under submerged conditions. Their production was found to depend on the developmental stage and treatment of the filamentous culture inoculum. A medium containing Bacto-peptone with a constant composition of amino acids was selected for the preparation of the inoculum. A two-week fermentation in a synthetic medium with mannitol at 24 +/- 1 degrees C resulted in an increased production of total alkaloids from the original value of 100-200 micrograms/mL to more than 2000 micrograms/mL. Addition of tryptophan did not further increase the production of alkaloids but resulted in changes of the spectrum of some metabolites, 2,3-Dihydroxybenzoic acid accompanied the alkaloids in the fermentation medium. alpha-Hydroxyethyllysergamide was the predominant component of extracellular alkaloids (80% in the first days of fermentation). During fermentation the level of this alkaloid continuously decreased while the concentration of the accompanying alkaloids, i.e. lysergamide and the corresponding minor isomers, increased.

• MeSH
• alkaloidy biosyntéza   MeSH
• Claviceps růst a vývoj   metabolismus   MeSH
• fermentace MeSH
• glukosa metabolismus   MeSH
• kultivační média MeSH
• kyselina lysergová analogy a deriváty   biosyntéza   MeSH
• mannitol metabolismus   MeSH
• tryptofan farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alkaloidy MeSH
• glukosa MeSH
• kultivační média MeSH
• kyselina lysergová MeSH
• mannitol MeSH
• tryptofan MeSH