Most cited article - PubMed ID 5691581
Tight coupling of monosaccharide transport and metabolism in Rhodotorula gracilis
- MeSH
- Acetamides pharmacology MeSH
- Acetates pharmacology MeSH
- Amides pharmacology MeSH
- Amino Acids metabolism MeSH
- Anaerobiosis MeSH
- Azides pharmacology MeSH
- Cycloheximide pharmacology MeSH
- Dinitrophenols pharmacology MeSH
- Fluorides pharmacology MeSH
- Glycine antagonists & inhibitors metabolism MeSH
- Iodoacetates pharmacology MeSH
- Caproates pharmacology MeSH
- Hydrogen-Ion Concentration MeSH
- Aspartic Acid antagonists & inhibitors metabolism MeSH
- Lysine antagonists & inhibitors metabolism MeSH
- Saccharomyces cerevisiae drug effects MeSH
- Saccharomyces drug effects metabolism MeSH
- Uranium pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acetamides MeSH
- Acetates MeSH
- Amides MeSH
- Amino Acids MeSH
- Azides MeSH
- Cycloheximide MeSH
- Dinitrophenols MeSH
- Fluorides MeSH
- Glycine MeSH
- Iodoacetates MeSH
- Caproates MeSH
- Aspartic Acid MeSH
- Lysine MeSH
- Uranium MeSH
Evidence for a mobile membrane carrier mediating the uphill monosaccharide transport in the yeastRhodotorula gracilis is based on two types of observations: (1) Countertransport was found with(14)C-labelledD-xylose,L-xylose,L-rhamnose and withL-rhamnose in a cell suspension preincubated with unlabelledD-xylose. This finding indicates, moreover, that both the hexoses and the pentose share the same membrane carrier. (2) The mobility of occupied carrier molecules is higher than that of free carrier molecules. This conclusion has been drawn from: (a) comparison of the initial rates of uptake of a labelled sugar into cells preincubated in the absence and in the presence of unlabelled sugar; (b) comparison on the half-saturation constant of transport with the dissociation constant of the sugar-carrier complex; and (c) comparison of the initial rates of efflux of a labelled sugar into sugar-free and sugar-containing medium.
- Publication type
- Journal Article MeSH
- MeSH
- Glucose metabolism MeSH
- Glycolysis MeSH
- Bicarbonates metabolism MeSH
- Carbon Isotopes MeSH
- Yeasts metabolism MeSH
- Lipids analysis MeSH
- Mitosporic Fungi metabolism MeSH
- Carbon Dioxide metabolism MeSH
- Pentosephosphates metabolism MeSH
- Oxygen Consumption MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glucose MeSH
- Bicarbonates MeSH
- Carbon Isotopes MeSH
- Lipids MeSH
- Carbon Dioxide MeSH
- Pentosephosphates MeSH
- MeSH
- Biological Transport, Active * MeSH
- Amides pharmacology MeSH
- Arabinose metabolism MeSH
- Candida drug effects metabolism MeSH
- Dinitrophenols pharmacology MeSH
- Fluorides pharmacology MeSH
- Glucose metabolism MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Monosaccharides metabolism MeSH
- Rhamnose metabolism MeSH
- Ribose metabolism MeSH
- Temperature MeSH
- Uranium pharmacology MeSH
- Xylose metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amides MeSH
- Arabinose MeSH
- Dinitrophenols MeSH
- Fluorides MeSH
- Glucose MeSH
- Monosaccharides MeSH
- Rhamnose MeSH
- Ribose MeSH
- Uranium MeSH
- Xylose MeSH