Most cited article - PubMed ID 5785597
The induction of auxotrophic mutants of Mycobacterium phlei PA by ultraviolet radiation
This review summarizes the main results obtained in the fields of general and molecular microbiology and microbial genetics at the Institute of Microbiology of the Academy of Sciences of the Czech Republic (AS CR) [formerly Czechoslovak Academy of Sciences (CAS)] over more than 50 years. Contribution of the founder of the Institute, academician Ivan Málek, to the introduction of these topics into the scientific program of the Institute of Microbiology and to further development of these studies is also included.
- MeSH
- Academies and Institutes history MeSH
- History, 20th Century MeSH
- Genetics, Microbial history MeSH
- Molecular Biology history MeSH
- Check Tag
- History, 20th Century MeSH
- Publication type
- Journal Article MeSH
- Historical Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Geographicals
- Czech Republic MeSH
New facts about the replication map of Mycobacterium phlei chromosome are summarized. Replication positions of two genes located in marginal regions of the replication map, ile close to the origin and ser near the terminus, were determined. Known positions of replication of some genes were defined with more precision within 2.5--5-min intervals using the method of sequential mutagenesis in synchronized cultures (leu, met, bac, pyr, stm, tet, cyc, his). Replication positions of genes responsible for the biosynthesis of thiamine and resistance to tetracycline and vancomycin were further identified. The contemporary replication map contains replication positions of 24 genes.
It was the aim of the present work to construct the replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by N-methyl-N-nitroso-N'-nitroguanidine in synchronously dividing populations and the method of analysis of gene frequency were applied. The order of replication of 19 genes on the chromosome was determined by means of induction of back mutations and forward mutations in auxotrophic mutants PA leu and PA met and in double auxotrophic mutants with methionine as a reference marker.
The aim of the present work was to construct a replication map of the chromosome of Mycobacterium phlei. The method of mutagenesis of the replication point by means of nitrosoguanidine was applied to synchronously multiplying populations. Back mutations and forward mutations were induced in auxotrophic mutants PA met and PA leu as well as in double auxotrophic mutants with methionine as the reference marker and the following order of replication of eleven genes on the chromosome was thus established: leu-Eth, Res-Stm, Cyk-pur-met, arg, Cyk-Bac-inl.
- MeSH
- Drug Resistance, Microbial MeSH
- Cell Division MeSH
- DNA, Bacterial biosynthesis MeSH
- Genes MeSH
- Leucine biosynthesis MeSH
- Chromosome Mapping * methods MeSH
- Methionine biosynthesis MeSH
- Methylnitronitrosoguanidine MeSH
- Mutation MeSH
- Mutagens MeSH
- Mycobacterium phlei * drug effects growth & development metabolism MeSH
- Mycobacterium * MeSH
- DNA Replication MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Leucine MeSH
- Methionine MeSH
- Methylnitronitrosoguanidine MeSH
- Mutagens MeSH
- MeSH
- Alkylating Agents pharmacology MeSH
- Drug Resistance, Microbial MeSH
- Isoniazid pharmacology MeSH
- Sulfonic Acids pharmacology MeSH
- Genetics, Microbial * MeSH
- Mutagens pharmacology MeSH
- Mycobacterium cytology drug effects MeSH
- Streptomycin pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Alkylating Agents MeSH
- Isoniazid MeSH
- Sulfonic Acids MeSH
- Mutagens MeSH
- Streptomycin MeSH
