Nejvíce citovaný článek - PubMed ID 61923
Suppression of photo-induced sporulation in Trichoderma viride by inhibitors
Nonsteroid antiinflammatory agents (NSAIA's) (inhibitors of cyclooxygenase and lipoxygenase) of several structural series inhibited growth of Trichoderma viride. The most potent growth inhibitors were indomethacin and its derivative repanidal which inhibited in the range of 0.1 mmol/L. The weakest inhibitors were acetylsalicylic acid and lysine salicylate which exerted only a weak effect at concentrations above 1 mmol/L. The inhibition of growth was accompanied by a stimulation of conidiation in the dark. A light pulse increased the efficiency of these drugs to stimulate the conidiation. Saccharomyces cerevisiae was as sensitive to NSAIA's as T. viride while Botrytis cinerea was less sensitive. The results indicate that arachidonate metabolism may play a role in the growth of fungi and may participate also in the process of conidiation.
- MeSH
- antiflogistika nesteroidní farmakologie MeSH
- buněčná diferenciace účinky léků MeSH
- druhová specificita MeSH
- mitosporické houby účinky léků fyziologie MeSH
- Saccharomyces cerevisiae účinky léků fyziologie MeSH
- spory hub MeSH
- Trichoderma účinky léků fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antiflogistika nesteroidní MeSH
Both natural and synthetic substances are conveniently used for studying metabolism, genetic aspects, morphogenesis of cell structures, life cycle and differentiation of fungi.
- MeSH
- antifungální látky farmakologie MeSH
- buněčná diferenciace MeSH
- houby účinky léků genetika metabolismus MeSH
- morfogeneze MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- antifungální látky MeSH
A total of 26 morphological and colour mutants of Trichoderma viride were characterized. They were divided into three groups based on morphology, pigmentation, growth rates and intensity of conidiation. Complementation analysis of colour mutants and mutants with disturbances in conidiation showed that after anastomosis and protoplast fusion only heterokaryotes are formed while no nuclear migration and diploidization takes place.
Growth and mutual interlacing of colonies of T. viride is affected by concentration of nutrients and presence of inhibitors in the culture medium. The most convenient colony restrictors were Bengal red, Ox Gall and sodium deoxycholate while L-sorbose and 2-deoxy-D-arabino-hexose were less efficient.
- MeSH
- kinetika MeSH
- kultivační média MeSH
- mitosporické houby genetika MeSH
- Trichoderma genetika růst a vývoj izolace a purifikace MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kultivační média MeSH
Numerous antibodies with a known mechanism of action are utilized as possible means for studying morphogenesis and differentiation. Inhibitors of biosynthesis of nucleic acids and proteins, compounds intervening with the synthesis and/or function of cell walls and membranes or compounds influencing the energy metabolism are particularly useful. The use of antibiotics for studies of the life cycle of viruses, bacteria, fungi, myxomycetes, protozoa and algae is analyzed in the present communication. Certain aspects of morphogenesis and functions of mitochondria and plastids were clarified with the aid of antibiotics. Relationships between production of antibiotics and differentiation of their producers are discussed in the final part of the paper.
- MeSH
- antibakteriální látky biosyntéza farmakologie MeSH
- Bacteria růst a vývoj MeSH
- chloroplasty fyziologie MeSH
- Eukaryota růst a vývoj MeSH
- houby růst a vývoj MeSH
- mitochondrie fyziologie MeSH
- morfogeneze MeSH
- organoidy fyziologie MeSH
- spory hub fyziologie MeSH
- viry růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- antibakteriální látky MeSH
The sporulating and nonsporulating mycelium of Trichoderma viride exhibited significant differences in the levels of triacylglycerols, sterol esters. fatty acids, gangliosides, phosphatidylethanolamine and sphingomyelin. Quantitative changes in the contents of polar and nonpolar lipids were observed also during the analyses of one-, two-, three-day-old sporulation zones in colonies subjected to repeated photo-induction. These findings are similar to the changes observed during the differentiation of other species of fungi.
After a short irradiation at 366 nm with 200 lx, the intensity of conidiation of Trichoderma viride colonies grown in the dark increased for the first 10 s proportionally with time. The increase slowed down after 10 s-5 min of exposure and after 10-60 min of irradiation the conidiation intensity began to decrease. When photo-induced by daylight, the conidiation started at a high rate after 25 h and persisted even after 48 h. The conidiation had no circadian character and its periodicity depended on the periodicity of photo-induction. Its intensity was also influenced by the carbon sources used, a maximum being reached with glucose (1-2%). Higher glucose concentrations inhibited conidiation but had no influence on growth of colonies.
- MeSH
- časové faktory MeSH
- kultivační média MeSH
- mitosporické houby fyziologie MeSH
- spory hub MeSH
- světlo * MeSH
- Trichoderma růst a vývoj fyziologie účinky záření MeSH
- ultrafialové záření MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kultivační média MeSH
The photo-induced conidiation of Trichoderma viride is suppressed by ethidium bromide, acriflavin, lomofungin and 8-quinolinol at concentrations which do not inhibit the colony growth of this deuteromycete.
- MeSH
- akridinová oranž farmakologie MeSH
- akriflavin farmakologie MeSH
- azaguanin farmakologie MeSH
- ethidium farmakologie MeSH
- fenaziny farmakologie MeSH
- flucytosin farmakologie MeSH
- fluorouracil farmakologie MeSH
- hydroxychinoliny farmakologie MeSH
- mitosporické houby fyziologie MeSH
- RNA antagonisté a inhibitory MeSH
- spory hub MeSH
- světlo * MeSH
- Trichoderma růst a vývoj fyziologie účinky záření MeSH
- vidarabin farmakologie MeSH
- virginiamycin farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- akridinová oranž MeSH
- akriflavin MeSH
- azaguanin MeSH
- ethidium MeSH
- fenaziny MeSH
- flucytosin MeSH
- fluorouracil MeSH
- hydroxychinoliny MeSH
- RNA MeSH
- vidarabin MeSH
- virginiamycin MeSH
