Nejvíce citovaný článek - PubMed ID 6310321
During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments. RNA-seq analysis of dbl2Δ transcriptome showed that the dbl2 deletion results in upregulation of more than 500 loci in Schizosaccharomyces pombe. Compared with the loci with no change in expression, the misregulated loci in dbl2Δ are closer to long terminal and long tandem repeats. Furthermore, the misregulated loci overlap with antisense transcripts, retrotransposons, meiotic genes and genes located in subtelomeric regions. A comparison of the expression profiles revealed that Dbl2 represses the same type of genes as the HIRA histone chaperone complex. Although dbl2 deletion does not alleviate centromeric or telomeric silencing, it suppresses the silencing defect at the outer centromere caused by deletion of hip1 and slm9 genes encoding subunits of the HIRA complex. Moreover, our analyses revealed that cells lacking dbl2 show a slight increase of nucleosomes at transcription start sites and increased levels of methylated histone H3 (H3K9me2) at centromeres, subtelomeres, rDNA regions and long terminal repeats. Finally, we show that other proteins involved in homologous recombination, such as Fbh1, Rad51, Mus81 and Rad54, participate in the same gene repression pathway.
- MeSH
- centromera MeSH
- histonový kód MeSH
- homologní rekombinace * MeSH
- nukleozomy metabolismus MeSH
- proteiny buněčného cyklu antagonisté a inhibitory metabolismus MeSH
- regulace genové exprese u hub * MeSH
- represorové proteiny fyziologie MeSH
- Schizosaccharomyces pombe - proteiny antagonisté a inhibitory metabolismus fyziologie MeSH
- Schizosaccharomyces genetika MeSH
- transkripční faktory antagonisté a inhibitory metabolismus MeSH
- umlčování genů * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Dbl2 protein, S pombe MeSH Prohlížeč
- hip1 protein, S pombe MeSH Prohlížeč
- nukleozomy MeSH
- proteiny buněčného cyklu MeSH
- represorové proteiny MeSH
- Schizosaccharomyces pombe - proteiny MeSH
- Slm9 protein, S pombe MeSH Prohlížeč
- transkripční faktory MeSH
Recombination is important for the repair of DNA damage and for chromosome segregation during meiosis; it has also been shown to participate in the regulation of cell proliferation. In the yeast Saccharomyces cerevisiae, recombination requires products of the RAD52 epistasis group. The Rad51 protein associates with the Rad51, Rad52, Rad54, and Rad55 proteins to form a dynamic complex. We describe a new strategy to screen for mutations which cause specific disruption of the interaction between certain proteins in the complex, leaving other interactions intact. This approach defines distinct protein interaction domains and protein relationships within the Rad51 complex. Alignment of the mutations onto the constructed three-dimensional model of the Rad51 protein reveal possible partially overlapping interfaces for the Rad51-Rad52 and the Rad51-Rad54 interactions. Rad51-Rad55 and Rad51-Rad51 interactions are affected by the same spectrum of mutations, indicating similarity between the two modes of binding. Finally, the detection of a subset of mutations within Rad51 which disrupt the interaction with mutant Rad52 protein but activate the interaction with Rad54 suggests that dynamic changes within the Rad51 protein may contribute to an ordered reaction process.
- MeSH
- DNA opravný a rekombinační protein Rad52 MeSH
- DNA primery genetika MeSH
- DNA vazebné proteiny chemie genetika metabolismus MeSH
- DNA-helikasy MeSH
- enzymy opravy DNA MeSH
- fungální proteiny chemie genetika metabolismus MeSH
- genetická epistáze MeSH
- geny hub MeSH
- methylmethansulfonát toxicita MeSH
- molekulární modely MeSH
- molekulární sekvence - údaje MeSH
- mutace MeSH
- oprava DNA genetika MeSH
- rekombinace genetická * MeSH
- rekombinasa Rad51 MeSH
- Saccharomyces cerevisiae - proteiny * MeSH
- Saccharomyces cerevisiae účinky léků genetika metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- techniky dvojhybridového systému MeSH
- teplota MeSH
- vazebná místa MeSH
- Publikační typ
- časopisecké články MeSH
- odvolaná publikace MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA opravný a rekombinační protein Rad52 MeSH
- DNA primery MeSH
- DNA vazebné proteiny MeSH
- DNA-helikasy MeSH
- enzymy opravy DNA MeSH
- fungální proteiny MeSH
- methylmethansulfonát MeSH
- RAD51 protein, S cerevisiae MeSH Prohlížeč
- RAD52 protein, S cerevisiae MeSH Prohlížeč
- RAD54 protein, S cerevisiae MeSH Prohlížeč
- RAD55 protein, S cerevisiae MeSH Prohlížeč
- rekombinasa Rad51 MeSH
- Saccharomyces cerevisiae - proteiny * MeSH
Effects of hyperosmotic stress on the transcriptional regulation of the HXT2 and HXT4 genes of Saccharomyces cerevisiae were investigated under glucose-repressed and -depressed growth conditions. Hyperosmotic stress repressed the transcription of these HXT genes up to 81% depending on growth conditions. Preconditioning of yeast cells for the hyperosmotic stress resulted in a much stronger repression of both HXT genes. The negative effect of hyperosmotic stress was much higher for HXT4 than HXT2. These results also show that hyperosmotic stress interferes with the glucose-dependent transcriptional activation or derepression of HXT2 and HXT4 genes transcription in S. cerevisiae.
- MeSH
- fungální proteiny genetika MeSH
- genetická transkripce MeSH
- geny hub * MeSH
- glukosa metabolismus MeSH
- membránové proteiny genetika MeSH
- osmotický tlak MeSH
- proteiny přenášející monosacharidy genetika MeSH
- proteiny usnadňující transport glukosy MeSH
- Saccharomyces cerevisiae - proteiny * MeSH
- Saccharomyces cerevisiae genetika růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fungální proteiny MeSH
- glukosa MeSH
- HXT2 protein, S cerevisiae MeSH Prohlížeč
- HXT4 protein, S cerevisiae MeSH Prohlížeč
- membránové proteiny MeSH
- proteiny přenášející monosacharidy MeSH
- proteiny usnadňující transport glukosy MeSH
- Saccharomyces cerevisiae - proteiny * MeSH