Gene manipulation in mycobacteria developed in two phases. In the first phase genes of mycobacteria were transferred into cells of E. coli and Streptomyces lividans. In the second phase, heterologous genes were transferred into mycobacteria either with a shuttle plasmid or hybrid plasmids. A prerequisite for successful gene manipulation in mycobacteria was a thorough understanding of plasmids in mycobacteria. Construction of recombinant DNA molecules contributed not only to the fact that mycobacteria did not remain outside the mainstream of modern genetic research but also to their present practical importance.

• MeSH
• Genes, Bacterial MeSH
• Escherichia coli genetics   MeSH
• Mycobacterium genetics   MeSH
• Plasmids MeSH
• Streptomyces genetics   MeSH
• Transfection MeSH
• Publication type
• Journal Article MeSH
• Review MeSH

Inducible pigmentation changes were observed in pigmented strains of Brevibacterium sp. M27 and B. flavum treated with N-methyl-N'-nitro-N-nitrosoguanidine. The highest frequency of induction was reached already at a survival of 30-40% with the maximal yield of 6-10%. As compared with the initial yellow colour, three new pigmentation types, viz. white, pink and orange, were observed. The yellow pigmented parent strains are most resistant to the lethal effects of UV radiation. By selecting pigmented mutants of all types on media containing antibiotics it was possible to obtain strains that were resistant either to tetracycline or to streptomycin. Auxotrophic pigmented mutants were also isolated. In multiple mutant strains of Brevibacterium sp. M27 a number of strainsexhibited a changed L-lysine production. In some strains the production was variable, whereasother strains did not produce L-lysine at all and stains with a limited production of other amino acids were also detected.

• MeSH
• Pigments, Biological metabolism   MeSH
• Brevibacterium drug effects   metabolism   radiation effects   MeSH
• Species Specificity MeSH
• Kinetics MeSH
• Methylnitronitrosoguanidine pharmacology   MeSH
• Mutation * MeSH
• Ultraviolet Rays MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Pigments, Biological MeSH
• Methylnitronitrosoguanidine MeSH