Escherichia coli is the common causative agent of urinary tract infections. Sixty-one strains of E. coli isolated from children with urinary tract infections were tested by colony hybridization for the presence of genes determining P and S fimbriae and hemolysin. Of these strains, 46 possess a gene for hemolysin, 44 for P fimbriae and 28 for S fimbriae. Only 30 strains formed lytic zones around the colonies on plates with sheep erythrocytes. The results indicated that simultaneous occurrence of genes in urinary E. coli was highest for P fimbriae and hemolysin and lower for other combinations of the tested genes.

• MeSH
• bakteriální fimbrie genetika   MeSH
• bakteriální geny * MeSH
• bakteriální proteiny genetika   MeSH
• dítě MeSH
• DNA sondy MeSH
• Escherichia coli genetika   izolace a purifikace   patogenita   MeSH
• hemolyziny genetika   MeSH
• hybridizace nukleových kyselin MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• infekce vyvolané Escherichia coli mikrobiologie   MeSH
• lidé MeSH
• proteiny z Escherichia coli * MeSH
• virulence genetika   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH
• DNA sondy MeSH
• hemolyziny MeSH
• Hlya protein, E coli MeSH Prohlížeč
• proteiny z Escherichia coli * MeSH

Escherichia coli was isolated from the urine of patients with pyelonephritis, with urinary tract infections other than pyelonephritis and with asymptomatic bacteriuria. Surface properties of the strains were analyzed by the salting-out aggregation test (SAT), hydrophobic interaction chromatography (HIC), Congo red binding (Crb), agglutination of erythrocytes (MRHA) and latex particles covered by digalactoside (PF) and by adherence to tissue culture cells. In addition, a DNA probe for the pap gene was used. The DNA probe detected the highest proportion of strains with pap gene in the group of patients with pyelonephritis, lower in the urinary tract infections other than pyelonephritis and the lowest in the group with asymptomatic bacteriuria. Tests for P-fimbriae (PF, MRHA) showed a similar distribution. Hydrophobicity measured by SAT and by HIC did not show differences among the tested groups of strains. The results suggest that factors other than the P-fimbriae and hydrophobicity may contribute to the persistence of E. coli in the urinary tract.

• MeSH
• bakteriální adheze genetika   fyziologie   MeSH
• bakteriální fimbrie fyziologie   MeSH
• bakteriální geny MeSH
• bakteriurie etiologie   mikrobiologie   MeSH
• disacharidy chemie   metabolismus   MeSH
• dítě MeSH
• DNA sondy MeSH
• Escherichia coli genetika   patogenita   fyziologie   MeSH
• hemaglutinace MeSH
• infekce močového ústrojí etiologie   mikrobiologie   MeSH
• infekce vyvolané Escherichia coli etiologie   mikrobiologie   MeSH
• latex fixační testy MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• pyelonefritida etiologie   mikrobiologie   MeSH
• receptory buněčného povrchu metabolismus   MeSH
• sacharidové sekvence MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 4-O-alpha-D-galactopyranosyl-D-galactose MeSH Prohlížeč
• disacharidy MeSH
• DNA sondy MeSH
• galactose receptor MeSH Prohlížeč
• receptory buněčného povrchu MeSH