Resistance to 17 antimicrobials, surface hydrophobicity, motility, biofilm, production of N-acylhomoserine lactone signal molecules (N-butyrylhomoserine lactone and N-3-oxolauroylhomoserine lactone) and response to oxidative stress were analyzed in 47 clinical Pseudomonas aeruginosa strains. In addition to natural resistance, the strains demonstrated the greatest level of resistance to cefotaxime (91.5%). Isolates in the range of 44.7-57.4% were resistant to aminoglycosides and ciprofloxacin, of 25.5-36.2% to cephalosporins. On the other hand, 97.9% remained susceptible to meropenem, 93.6% to piperacillin + tazobactam and 87.2% to piperacillin. The majority of the strains (72.3%) manifested their hydrophilic character. Higher zones of motility showed 12 isolates (in average 54.8 mm) as compared to the others (30.2 mm). Approximately 1/3 of the strains (29.8%) produced a higher amount of biofilm quantified by measuring the absorbance of solubilized crystal violet (0.20-0.46) than the rest of isolates (0-0.19). All but two strains produced N-3-oxolauroylhomoserine lactone and in 48.9% of samples N-butyrylhomoserine lactone were detected. Only four isolates with higher biofilm production showed both types of homoserine lactone. Majority of the strains (70.2%) manifested higher resistance to H2O2 than the rest of the strains. The group of strains resistant to aminoglycosides and ciprofloxacin revealed a significantly higher number of hydrophobic strains (compared with the sensitive ones). In contrast, higher number of strains sensitive to aminoglycosides and ciprofloxacin or only to ciprofloxacin produced N-butyrylhomoserine lactone and biofilm (compared to the resistant ones). Such association was not found among the rest of the tested parameters. The results indicate that the resistance to antimicrobials in P. aeruginosa isolates was not generally associated with changes in the production of the pathogenicity factors.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• biofilmy růst a vývoj   MeSH
• fenotyp MeSH
• fyziologická adaptace MeSH
• gama-butyrolakton analogy a deriváty   biosyntéza   MeSH
• genciánová violeť metabolismus   MeSH
• hydrofobní a hydrofilní interakce MeSH
• lidé MeSH
• oxidační stres MeSH
• oxidancia farmakologie   MeSH
• peroxid vodíku farmakologie   MeSH
• pohyb MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa účinky léků   izolace a purifikace   patogenita   MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• gama-butyrolakton MeSH
• genciánová violeť MeSH
• oxidancia MeSH
• peroxid vodíku MeSH

Potential virulence factors (elastase, proteinase, lipase, phospholipase C, alginate) as well as surface properties (hydrophobicity, motility) were determined in 103 Pseudomonas aeruginosa strains isolated from patients with cancer. Nontypable strains were the dominant group (60%), followed by serotypes O11 (17%), O12 (7%) and O4 (5%). Seventy-one strains (69%) produced high level of elastase (10-60 mg/L), 87% of the strains possessed high activity of proteinase (bacterial) (10-250 mg/L) and 69% of the strains demonstrated higher level of lipase (20-150 U/mL); these elevated levels of enzymes were associated mainly with nontypable strains. On the other hand, 79% of the strains did not produce or produced only a low level of phospholipase C and 60% of isolates did not manifest any or very low production of alginate. Hydrophobicity demonstrated by adherence of the bacteria to xylene was shown by 69% of strains; 94% of strains aggregated with ammonium sulfate. Motility in the range of 31-80 mm was found in 76 strains (74%). The considerable virulence of tested P. aeruginosa strains was confirmed. The nontypable strains manifested the most frequent group with high level of elastase, proteinase, lipase, hydrophobicity and motility.

• MeSH
• algináty metabolismus   MeSH
• bakteriální adheze MeSH
• endopeptidasy biosyntéza   MeSH
• fosfolipasy typu C biosyntéza   MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• lidé MeSH
• lipasa biosyntéza   MeSH
• nádory komplikace   mikrobiologie   MeSH
• oportunní infekce komplikace   mikrobiologie   MeSH
• pankreatická elastasa biosyntéza   MeSH
• pseudomonádové infekce komplikace   mikrobiologie   MeSH
• Pseudomonas aeruginosa klasifikace   izolace a purifikace   patogenita   fyziologie   MeSH
• sérotypizace MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• algináty MeSH
• endopeptidasy MeSH
• fosfolipasy typu C MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• lipasa MeSH
• pankreatická elastasa MeSH

Thirty-three uropathogenic strains of Pseudomonas aeruginosa were investigated for hemolytic activity in both bacterial broth culture filtrates and isolate lyzates, resistance to bactericidal activity of fresh human serum, resistance to six antibiotics and plasmid DNA profile. Twenty-four of the 33 (73%) bacterial filtrates showed lysis of rabbit erythrocytes, as did the three after guinea-pig erythrocyte treatment. Twelve of 33 isolate lysates showed in parallel lysis of both types of erythrocytes used. Serum resistance was found in 17 (52%) isolates, intermediate resistance in 15 (45 %) isolates and only one isolate showed serum sensitivity. Resistance to antibiotics was detected as follows (in %): tetracycline 94, kanamycin 79, chloramphenicol 76, septrin 73, ampicillin 64, streptomycin 45, gentamicin 18. None of the isolates investigated showed resistance to colistine. With the exception of one isolate, plasmid DNA was detected in all P. aeruginosa strains.

• MeSH
• antibiotická rezistence MeSH
• baktericidní aktivita krve MeSH
• dítě MeSH
• dospělí MeSH
• hemolýza MeSH
• hemolyziny biosyntéza   MeSH
• infekce močového ústrojí mikrobiologie   MeSH
• králíci MeSH
• lidé MeSH
• morčata MeSH
• plazmidy genetika   izolace a purifikace   MeSH
• předškolní dítě MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa genetika   izolace a purifikace   patogenita   MeSH
• techniky in vitro MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• králíci MeSH
• lidé MeSH
• morčata MeSH
• předškolní dítě MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hemolyziny MeSH